为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Neuroscience Cell Type Marker Neuron marker Soma marker
使用敲除细胞株进行验证

Anti-EEA1抗体- Early Endosome Marker (ab2900)

  • Datasheet
  • SDS
Reviews (37)Q&A (23)References (182)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)

Key features and details

  • Rabbit polyclonal to EEA1 - Early Endosome Marker
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Dog, Human, Xenopus laevis, Chinese hamster
  • Isotype: IgG

你可能会感兴趣

一抗
Product image
Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
一抗
Product image
Anti-Rab5 antibody - Early Endosome Marker (ab18211)
分析检测
Product image
HDL Uptake Assay Kit (Fluorometric) (ab204717)

查看更多相关产品

概述

  • 产品名称

    Anti-EEA1抗体- Early Endosome Marker
    参阅全部 EEA1 一抗
  • 描述

    兔多克隆抗体to EEA1 - Early Endosome Marker
  • 宿主

    Rabbit
  • 特异性

    Detects a band at 180kDa that represents EEA1 in Western blotting on human cell lines (corresponds to results seen in Mu et al). Also detects a band at 100kDa, we are unsure as to the identity of this band. Immunofluorescence staining of EEA1 in HeLa cells yields a punctate staining pattern consistent with the cytoplasmic distribution of endosomes.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    Human
    WB
    Mouse
    Rat
    Dog
    Human
    Xenopus laevis
    Chinese hamster
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human EEA1 aa 1350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab14946)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Endosome
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Regulation

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human EEA1 protein (ab112379)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-EEA1 antibody [1G11] - Early Endosome Marker (ab70521)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Rat
Human
WB
Mouse
Rat
Dog
Human
Xenopus laevis
Chinese hamster
All applications
Chicken
Hamster
Cow
Zebrafish
Rhesus monkey
Aplysia
应用 Ab评论 说明
ICC/IF (23)
Use a concentration of 1 µg/ml.
WB (11)
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 160 kDa).

Abcam recommends using milk as the blocking agent.

说明
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 160 kDa).

Abcam recommends using milk as the blocking agent.

靶标

  • 功能

    Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
  • 序列相似性

    Contains 1 C2H2-type zinc finger.
    Contains 1 FYVE-type zinc finger.
  • 结构域

    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 细胞定位

    Cytoplasm. Early endosome membrane.
  • Target information above from: UniProt accession Q15075 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8411 Human
    • Entrez Gene: 216238 Mouse
    • Entrez Gene: 314764 Rat
    • Omim: 605070 Human
    • SwissProt: Q15075 Human
    • SwissProt: Q8BL66 Mouse
    • Unigene: 567367 Human
    • Unigene: 210035 Mouse
    • Unigene: 23513 Rat
    see all
  • 别名

    • Early endosome antigen 1 antibody
    • Early endosome antigen 1, 162kD antibody
    • Early endosome associated protein antibody
    • EEA 1 antibody
    • EEA1 antibody
    • EEA1_HUMAN antibody
    • Endosome associated protein p162 antibody
    • Endosome-associated protein p162 antibody
    • MST105 antibody
    • MSTP105 antibody
    • ZFYVE2 antibody
    • Zinc finger FYVE domain containing protein 2 antibody
    • Zinc finger FYVE domain-containing protein 2 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)

    ICC/IF image of ab2900 stained human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2900, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: EEA1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: NIH3T3 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab2900 observed at 162 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab2900 was shown to recognize EEA1 in wild-type HAP1 cells as signal was lost at the expected MW in EEA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and EEA1 knockout samples were subjected to SDS-PAGE. Ab2900 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : HEK-293 whole cell lysate
    Lane 6 : NIH 3T3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 160 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin (Lanes 1-3) or 3% Milk (Lanes 4-6) before being incubated with ab2900 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti rabbit at 1/50000 dilution

    Performed under reducing conditions.

    Predicted band size: 160 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 41 kDa, 50 kDa. We are unsure as to the identity of these extra bands.



    Fluorescence detection of secondary antibody.
  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

    Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of rabbit polyclonal to EEA1 (ab2900, 1/200; Alexa Fluor 488 1/200; green) and monoclonal mouse anti-β.

  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

    ab2900 Rabbit polyclonal to EEA1 (1/500) was used in fixed HEK cells that had been transfected with Rab5-GFP. Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling rabbit polyclonal to EEA1 ab2900 (1/500; Alexa Fluor® 568; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 5µm. Showing little co-localisation as expected.

  • Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody - Early Endosome Marker (ab2900)This image is courtesy of an anonymous Abreview

    ab2900 staining mouse L-cells by ICC/IF.  Cells were formaldehyde fixed, permeabilized in Triton X-100 and incubated with ab2900 diluted 1/1000 for 1 hour at 37°C.  A Cy2® conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HEK293 Whole Cell lysate with Human EEA1 peptide (ab14946) at 1 µg

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 160 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Lane 1 - 2 : EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution, HEK293 Whole Cell lysate at 20 ug Lane 1 : as above Lane 2 : EEA1 peptide (ab14946) at 1 ug Secondary Goat polyclonal to Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution Performed under reducing conditions. Predicted band size : 160kD Observed band size : 180kD (why is the actual band size different from the predicted?)
  • Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    Western blot - Anti-EEA1 antibody - Early Endosome Marker (ab2900)
    All lanes : Anti-EEA1 antibody - Early Endosome Marker (ab2900) at 1 µg/ml

    Lane 1 : Xenopus laevis lysate
    Lane 2 : Mouse 3T3 cell lysate
    Lane 3 : Mouse brain cell lysate
    Lane 4 : Mouse liver cell lysate
    Lane 5 : Rat brain cell lysate
    Lane 6 : Rat liver cell lysate
    Lane 7 : Dog lysate
    Lane 8 : CHO cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 160 kDa
    Observed band size: 100,180 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    The Western blot shows that ab14944 reacts strongly with mouse 3T3 and CHO cell lysates. Weak cross-reactivity is seen with Xenopus, mouse brain, mouse liver, rat brain and dog lysates. The antibody does not appear to cross-react with rat liver lysate.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (182)

    发表研究结果有使用 ab2900?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2900 被引用在 182 文献中.

    • Xue J  et al. Addition of High Molecular Weight Hyaluronic Acid to Fibroblast-Like Stromal Cells Modulates Endogenous Hyaluronic Acid Metabolism and Enhances Proteolytic Processing and Secretion of Versican. Cells 9:N/A (2020). PubMed: 32668663
    • Naffa R  et al. P38 MAPK Promotes Migration and Metastatic Activity of BRAF Mutant Melanoma Cells by Inducing Degradation of PMCA4b. Cells 9:N/A (2020). PubMed: 32414111
    • Beach R  et al. GJB2 Mutations Linked to Hearing Loss Exhibit Differential Trafficking and Functional Defects as Revealed in Cochlear-Relevant Cells. Front Cell Dev Biol 8:215 (2020). PubMed: 32300592
    • Salgado-Polo F  et al. Sequence-dependent trafficking and activity of GDE2, a GPI-specific phospholipase promoting neuronal differentiation. J Cell Sci 133:N/A (2020). PubMed: 31932507
    • Actis Dato V  et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer Cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Cancer Cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 16 2018

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Pancreas or PDAC cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Pancreas or PDAC cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 14 2016

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (BRAIN)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Treatment
    alpha synuclein
    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 09 2015

    Western blot abreview for Anti-EEA1 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (Hela)
    Specification
    Hela
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 12 2013

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Specification
    fibroblast
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 21 2013

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rhesus monkey Cell lysate - whole cell (Rhesus monkey kidney cells (FRhK-4))
    Loading amount
    20 µg
    Specification
    Rhesus monkey kidney cells (FRhK-4)
    Gel Running Conditions
    Reduced Denaturing (7 %)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 19 2012

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK 293T)
    Loading amount
    20 µg
    Specification
    HEK 293T
    Gel Running Conditions
    Reduced Denaturing (10% Bis Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Brian Koss

    Verified customer

    提交于 Dec 01 2011

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (SH-SY5Y-wild type cells)
    Loading amount
    8 µg
    Specification
    SH-SY5Y-wild type cells
    Gel Running Conditions
    Reduced Denaturing (10-20% tricine)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 11 2011

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Non-small cell lung carcinoma)
    Loading amount
    20 µg
    Specification
    Non-small cell lung carcinoma
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 01 2010

    Western blot abreview for Anti-EEA1 antibody - Early Endosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lung)
    Loading amount
    30 µg
    Specification
    lung
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 30 2009

    1-10 of 11 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有