Anti-EDD抗体(ab70311)
Key features and details
- Rabbit polyclonal to EDD
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-EDD抗体
参阅全部 EDD 一抗 -
描述
兔多克隆抗体to EDD -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to a region between residues 600 and 650 of human EDD. (NP_056986.2 )
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阳性对照
- HeLa and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab70311 was affinity purified using an epitope specific to EDD immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 309 kDa (predicted molecular weight: 309 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). May be involved in maturation and/or transcriptional regulation of mRNA. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. -
组织特异性
Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 PABC domain.
Contains 1 UBR-type zinc finger. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 515582 Cow
- Entrez Gene: 51366 Human
- Entrez Gene: 70790 Mouse
- Entrez Gene: 100157625 Pig
- Entrez Gene: 117060 Rat
- Omim: 608413 Human
- SwissProt: O95071 Human
- SwissProt: Q80TP3 Mouse
see all -
别名
- 4432411E13Rik antibody
- AW549941 antibody
- C77315 antibody
see all
图片
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All lanes : Anti-EDD antibody (ab70311) at 0.4 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 309 kDa
Exposure time: 3 minutes -
Immunoprecipitation of HeLa cells using ab70311
Lane 1: ab70311 current lot
Lane 2: ab70311 previous lot
Lane 3: Control IgG
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of mouse plasmacytoma staining EDD with ab70311 at 1/1000 dilution
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded sections of human lung carcinoma staining EDD with ab70311 at 1/1000 dilution
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab70311 被引用在 10 文献中.
- Wang M et al. Targeting UBR5 in hepatocellular carcinoma cells and precise treatment via echinacoside nanodelivery. Cell Mol Biol Lett 27:92 (2022). PubMed: 36224534
- Wang K et al. UBR5 regulates proliferation and radiosensitivity in human laryngeal carcinoma via the p38/MAPK signaling pathway. Oncol Rep 44:685-697 (2020). PubMed: 32468011
- Duan Z et al. TMT-based quantitative proteomics analysis reveals the attenuated replication mechanism of Newcastle disease virus caused by nuclear localization signal mutation in viral matrix protein. Virulence 11:607-635 (2020). PubMed: 32420802
- Khan MM et al. Protein interactome of the Cancerous Inhibitor of protein phosphatase 2A (CIP2A) in Th17 cells. Curr Res Immunol 1:10-22 (2020). PubMed: 33817627
- Ding F et al. UBR5 oncogene as an indicator of poor prognosis in gastric cancer. Exp Ther Med 20:7 (2020). PubMed: 32934672
- Xie Z et al. Significance of the E3 ubiquitin protein UBR5 as an oncogene and a prognostic biomarker in colorectal cancer. Oncotarget 8:108079-108092 (2017). PubMed: 29296225
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Liao L et al. E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer. Cancer Res 77:2090-2101 (2017). PubMed: 28330927
- Bolt MJ et al. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-a protein levels and activity. Oncogene N/A:N/A (2014). Human . PubMed: 24441042
- Morrison J et al. Dengue virus co-opts UBR4 to degrade STAT2 and antagonize type I interferon signaling. PLoS Pathog 9:e1003265 (2013). WB ; Human . PubMed: 23555265