Anti-ECHS1抗体(ab228631)
Key features and details
- Rabbit polyclonal to ECHS1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ECHS1抗体
参阅全部 ECHS1 一抗 -
描述
兔多克隆抗体to ECHS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human ECHS1 (internal sequence). The exact sequence is proprietary.
Database link: P30084 -
阳性对照
- WB: Mouse brain tissue whole cell lysate; HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract. IHC-P: Mouse heart, ovary and liver tissue. ICC/IF: A431 and HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228631于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/10000. Predicted molecular weight: 31 kDa.
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IP |
1/100 - 1/500.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/10000. Predicted molecular weight: 31 kDa. |
IP
1/100 - 1/500. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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相关性
ECHS1 (Enoyl Coenzyme A hydratase, short chain, 1) catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs, in the second step of the mitochondrial fatty acid beta-oxidation pathway. -
细胞定位
Mitochondrion matrix. -
数据库链接
- Entrez Gene: 1892 Human
- Entrez Gene: 93747 Mouse
- Omim: 602292 Human
- SwissProt: P30084 Human
- SwissProt: Q8BH95 Mouse
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别名
- C80529 antibody
- EC 4.2.1.17 antibody
- ECHS 1 antibody
see all
图片
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A431 (human epidermoid carcinoma cell line) cells stained for ECHS1 (green) using ab228631 at 1/200 dilution in ICC/IF. Cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 minutes.
Blue: Hoechst 33342 staining.
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Paraffin-embedded mouse liver tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Anti-ECHS1 antibody (ab228631) at 1/1000 dilution + Mouse brain tissue whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 31 kDa12% SDS-PAGE
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All lanes : Anti-ECHS1 antibody (ab228631) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa12% SDS-PAGE
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ECHS1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 μg ab228631. Western blot was performed from the immunoprecipitate using ab228631.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab228631 in HEK-293T whole cell extract.
Lane 3: ab228631 IP in HEK-293T whole cell extract.
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Paraffin-embedded mouse heart tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse ovary tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse liver tissue stained for ECHS1 with ab228631 at 1/500 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ECHS1 (green) using ab228631 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab228631 尚未被引用在任何文献中。