Anti-EBV Latent膜蛋白1抗体[CS1，CS2，CS3，CS4]，prediluted (ab7502)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted (ab7502)

    Key features and details

    • Mouse monoclonal [CS1, CS2, CS3, CS4] to EBV Latent Membrane Protein 1, prediluted
    • Suitable for: IHC-P, IHC-Fr
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-EBV Latent膜蛋白1抗体[CS1，CS2，CS3，CS4]，prediluted
      参阅全部 EBV Latent Membrane Protein 1 一抗

    • 描述

      小鼠单克隆抗体[CS1，CS2，CS3，CS4] to EBV Latent膜蛋白1，prediluted

    • 宿主

      Mouse

    • 特异性

      This antibody is specific to 60 kD latent membrane protein (LMP1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic c-terminus of the cytoplasmic domain of LMP1. This antibody stains strongly with EBV positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis.

    • 经测试应用

      适用于: IHC-P, IHC-Frmore details

    • 种属反应性

      EBV.

    • 免疫原

      Recombinant fusion protein containing sequences of bacterial beta-galactosidase and EBV encoded Latent membrane protein.

    • 阳性对照

      • Infected lymphoblastoma.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7502于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr
    Use at an assay dependent concentration.

    靶标

    • 相关性

      EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.

    • 别名

      • BNLF1 antibody
      • EBV antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      • Latent membrane protein 1 antibody
      • LMP1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted (ab7502)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted (ab7502)

      The image shows lung tissue diagnosed with Post-Transplantation Lymphoproliferative Disorder (PTLD). Mononuclear cells are stained brown with DAB chromogen around the cytoplasm - typical for Latent Membrane Protein-1 of EBV.

      This picture was kindly supplied as part of the review submitted by Pradeep Rai.

    文献 (2)

    发表研究结果有使用 ab7502？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7502 被引用在 2 文献中.

    • Li C  et al. EBNA2-deleted Epstein-Barr virus (EBV) isolate, P3HR1, causes Hodgkin-like lymphomas and diffuse large B cell lymphomas with type II and Wp-restricted latency types in humanized mice. PLoS Pathog 16:e1008590 (2020). PubMed: 32542010
    • Nawandar DM  et al. Differentiation-Dependent LMP1 Expression Is Required for Efficient Lytic Epstein-Barr Virus Reactivation in Epithelial Cells. J Virol 91:N/A (2017). PubMed: 28179525

    客户评价及客户问答

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    Question

    Good afternoon, I am using this antibody to detect EBV in ganglionar tissue, but, i haven´t seen any reaction yet. I´ve been using HEAT treatment with citrate buffer for the antigen retrieval step. I´ve seen in your website you have a picture of an IHC for EBV virus, could you send me the reference to check the IHC protocol, so i could reach the same reaction? Also which tissue you are using? Thanks a lot

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    Answer

    Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

    What was your order number? I do not see any orders to Peru in my records.

    The IHC-P image on the datasheet is from a customer's testing. The image shows lung tissue diagnosed with Post-Transplantation Lymphoproliferative Disorder (PTLD). Mononuclear cells are stained brown with DAB chromogen around the cytoplasm - typical for Latent Membrane Protein-1 of EBV. You can see his basic protocol linked below and you can contact him by clicking on his name online.

    https://www.abcam.com/EBV-Latent-Membrane-Protein-1-antibody-CS1-CS2-CS3-CS4-prediluted-ab7502.html&tab=abreviews&intabreviewid=2103

    I have some references listed here from the lab, but I'm unsure if they are background articles or cite this antibody in particultar: The lab recommends infected lymphoblastoma as a positive control, and the references are below.

    1) Jarrett et al. J Clin Pathol 44: 844, 1991.
    2) Murray et al. J Pathol 166:1, 1992.
    3) Pallesen et al. Lancet 337: 320, 1991.
    4) Rowe et al. J Gene Virol 68: 1575, 1987.

    The lab also recommends following this protocol:

    http://www.jove.com/pdf/308/jove-protocol-308-immunohistochemistry-paraffin-sections-using-vectastain-abc-kit-from?ID=308

    Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

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    Question

    I would like to know if there is a MSDS for this product ab7502, specially HAZMAT and DOT part, because i need to make an international request.

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    Answer

    Thank you for contacting us. This antibody is non-hazardous. Please find the requested MSDS for non-hazardous products attached. Let me know if you need any additional information or assistance.

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    Question

    What is the sequence of the fusion protein this antibody was raised to?

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    Answer

    Thank you for your enquiry. Unfortunately this information was not released by the originator of the antibody. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

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    Question

    Do you have an approximate antibody concentration for this product.

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    Answer

    The approximate concentration of ab7502 is 8 ug/ml.

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