Anti-EBV gp340/220 Envelope蛋白抗体[10B5] (ab6525)

Submit a review Q&A (3)References (1)

Key features and details

  • Mouse monoclonal [10B5] to EBV gp340/220 Envelope Protein
  • Suitable for: ELISA, ICC/IF
  • Isotype: IgG1

概述

  • 产品名称

    Anti-EBV gp340/220 Envelope蛋白抗体[10B5]

  • 描述

    小鼠单克隆抗体[10B5] to EBV gp340/220 Envelope蛋白

  • 宿主

    Mouse

  • 特异性

    This antibody is specific for Epstein-Barr Virus.

  • 经测试应用

    适用于: ELISA, ICC/IFmore details

  • 种属反应性

    与反应: Other species

性能

应用

Our Abpromise guarantee covers the use of ab6525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/800.
ICC/IF Use at an assay dependent dilution.

靶标

  • 相关性

    Epstein-Barr virus is a member of the herpesvirus family and one of the most common human viruses. The envelope glycoprotein Gp340/Gp220 is the most abundant vomponent of the viral envelope and is believed to be responsible for EBV binding to CR2 receptor on human B-Cells.

  • 细胞定位

    Virion membrane: most abundant component of the viral envelope.

  • 别名

    • BLLF1a antibody
    • Envelope glycoprotein Gp220/340 antibody
    • Envelope glycoprotein GP340/220 antibody
    • Envelope glycoprotein GP340/GP220 antibody
    • Epstein Barr gp350 envelope protein antibody
    • Epstein Barr virus antibody
    • Epstein Barr Virus envelope glycoprotein complex 250/350 antibody
    • gp 350 antibody
    • Gp350 antibody
    • MA antibody
    • Membrane antigen antibody
    see all

文献 (1)

发表研究结果有使用 ab6525？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6525 被引用在 1 文献中.

  • Watanabe R  et al. Inhibition of NF-?B activation by a novel IKK inhibitor reduces the severity of experimental autoimmune myocarditis via suppression of T-cell activation. Am J Physiol Heart Circ Physiol 305:H1761-71 (2013). PubMed: 24097428

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Question

many thanks for Your detailed reply! I already know the publication that You cite, and I have also considered to try Sodium Butyrate to superinduce the lytic cycle. I did not do it yet as one of my Professors told me that the "baseline activity" of P3-HR1 cells, the proportion of virus-producing cells when not stimulated, should be approximately 20%. (I could not find any literature on this...). If true, in my FITC assay with 10.000 cells per slide there would have been ~2000 virus producing cells, which should be far enough. The fact that the virus load in the P3-HR1 cell culture supernatant is 10E5 to 10E6 per ml (2 days after passage) also supports the presumption that I have enough EBV-producing cells. Anyway, I will go on working on this assay and I will re-consider the approach You proposed. Thank You for Your efforts! If I may I will contact You again, to report success or ask You another question. I wish You a Happy New Year!

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Answer

Thank you for your enquiry. Yes, you are right. Given this rationale I would expect a positive signal. However, it may be useful to superinduce your cells if you obtaining no signal whatsoever. Could you also perform immunofluorescence to detect the presence of EBV in your cells? Good luck with your experiments!

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Question

may I ask You for further advice concerning ab6525? I am using this antibody as primary antibody in an antigen ELISA to detect Epstein-Barr Virus producing B-Cells / Virus particles, which doesn't work. I have tried several dilutions of this antibody but did not get a positive result until now. In theory - in my view - this ab should be the right choice, as literature says that gp350/220 is the most abundant viral protein in the lytically infected cell plasma membrane and in the virus envelope. I have tested the antibody using P3-HR1 cells (lymphoblastoid producer cell line which spontaneously release virus particles) and FITC-labelled anti-mouse antibodies: there was no fluorescence (only some unspecific fluorescence). Maybe You can help me?! I would be grateful for any information that might help to solve the problem!

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Answer

Thank you for your enquiry. I am sorry for the delay in a response to your enquiry. I have performed a literature search and it seems that your approach is certainly suitable for the detection of EBV: http://www.clinchem.org/cgi/reprint/50/10/1814 In the above publication they superinduced the lytic cycle by the use of the histone deacetylase inhibitor Sodium Butyrate. I was wondering whether you had considered this approach to get a firm positive control from your P3HR1 cells: "gp350/220 mRNAs was quantified in the EBV-infected cell lines P3HR1, B95-8, and Akata. The P3HR1 and B95-8 B-cell lines spontaneously release viral particles. Moreover, the EBV lytic cycle can be superinduced by treatment with 30 ug/L 12-tetradecanoylphorbol 13-acetate and 3 mmol/L sodium butyrate" We do have some excellent ELISA protocols available as PDF files at the following location. I was wondering whether you were using a similar approach: http://ops.abcam.com/index.html?pageconfig=resource&rid=10417 If you continue to have difficulties with this antibody I would appreciate it if you could complete our on line technical questionnaire by clicking on the following link. This will better enable our technical team to determine the steps that you have taken to optimise this antibody. https://www.abcam.com/index.html?section=elisa&pageconfig=technical&intAbID=6525&mode=questionaire I look forward to hearing from you.

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Question

May I ask for some additional information concerning the product ab6525 (Mouse monclonal to EBV pg350 Envelope Protein): For ELISA, You recommend the dilution " ELISA Titer 1+@ 1:200 " Could You please explain what this exactly means or give the recommended dilution (range) in microgramm/ml? Thanks for Your help. Best regards,

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Answer

Thank you for your enquiry. The information on our datasheet was slightly incorrect. I have updated the information to include the ELISA dilution information :ELISA Titer: 0.100 @ >1:800. The nomenclature that is employed here refers to the dilution at which an absorbance of 0.1 is achieved. Therefore in this case an absorbance of 0.1 was generated using a dilution of 1:800 or more. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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