Anti-EBP50/NHERF-1抗体(ab3452)
Key features and details
- Rabbit polyclonal to EBP50/NHERF-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-EBP50/NHERF-1抗体
参阅全部 EBP50/NHERF-1 一抗 -
描述
兔多克隆抗体to EBP50/NHERF-1 -
宿主
Rabbit -
特异性
Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Human
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免疫原
Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
Sequence:RSASSDTSEELNSQDSP
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| IHC-P |
Mouse
|
| WB |
Human
|
| All applications |
Rabbit
Cow
Cynomolgus monkey
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
| IHC-P |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
|
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC. -
组织特异性
Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. -
疾病相关
Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization). -
序列相似性
Contains 2 PDZ (DHR) domains. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells. - Information by UniProt
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数据库链接
- Entrez Gene: 505242 Cow
- Entrez Gene: 9368 Human
- Entrez Gene: 26941 Mouse
- Entrez Gene: 100009196 Rabbit
- Omim: 604990 Human
- SwissProt: Q3SZK8 Cow
- SwissProt: O14745 Human
- SwissProt: P70441 Mouse
see all -
别名
- EBP 50 antibody
- EBP50 antibody
- Ezrin radixin moesin binding phosphoprotein 50 antibody
see all
图片
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Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution
All lanes : Whole cell lysate prepared from human HepG2 cells
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 40 seconds
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Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution
All lanes : Whole tissue lysate prepared from mouse liver
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 30 seconds
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Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.
文献 (33)
ab3452 被引用在 33 文献中.
- Tsurudome Y et al. Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function. Sci Rep 8:9072 (2018). PubMed: 29899468
- Benhamouche-Trouillet S et al. Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis. Development 145:N/A (2018). PubMed: 29712669
- Kurtenbach S et al. The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golfto Olfactory Cilia. Sci Rep 7:8409 (2017). PubMed: 28814779
- Vogl C et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep 18:2015-2029 (2017). PubMed: 28893864
- Datta A et al. Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibition. Cell Signal 40:91-98 (2017). PubMed: 28888686
