为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Tags & Cell Markers Cell Type Markers Epi / Endo-thelial

Anti-EBP50/NHERF-1抗体(ab3452)

  • Datasheet
  • SDS
Reviews (8)Q&A (2)References (33)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)

Key features and details

  • Rabbit polyclonal to EBP50/NHERF-1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

你可能会感兴趣

染色
Product image
Copper Stain Kit (ab150666)
一抗
Product image
Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-EBP50/NHERF-1抗体
    参阅全部 EBP50/NHERF-1 一抗
  • 描述

    兔多克隆抗体to EBP50/NHERF-1
  • 宿主

    Rabbit
  • 特异性

    Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein.
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data
  • 免疫原

    Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
    Sequence:

    RSASSDTSEELNSQDSP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Epi / Endo-thelial
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Mouse
WB
Human
All applications
Rabbit
Cow
Cynomolgus monkey
应用 Ab评论 说明
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
Use a concentration of 10 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P
Use a concentration of 10 µg/ml.

靶标

  • 功能

    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
  • 组织特异性

    Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
  • 疾病相关

    Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
  • 序列相似性

    Contains 2 PDZ (DHR) domains.
  • 翻译后修饰

    Phosphorylated on serine residues.
  • 细胞定位

    Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Target information above from: UniProt accession O14745 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 505242 Cow
    • Entrez Gene: 9368 Human
    • Entrez Gene: 26941 Mouse
    • Entrez Gene: 100009196 Rabbit
    • Omim: 604990 Human
    • SwissProt: Q3SZK8 Cow
    • SwissProt: O14745 Human
    • SwissProt: P70441 Mouse
    • SwissProt: Q28619 Rabbit
    • Unigene: 724482 Human
    • Unigene: 728760 Human
    • Unigene: 27842 Mouse
    see all
  • 别名

    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

图片

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole cell lysate prepared from human HepG2 cells

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 40 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole tissue lysate prepared from mouse liver

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)

    Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (33)

    发表研究结果有使用 ab3452?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3452 被引用在 33 文献中.

    • Tsurudome Y  et al. Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function. Sci Rep 8:9072 (2018). PubMed: 29899468
    • Benhamouche-Trouillet S  et al. Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis. Development 145:N/A (2018). PubMed: 29712669
    • Kurtenbach S  et al. The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golfto Olfactory Cilia. Sci Rep 7:8409 (2017). PubMed: 28814779
    • Vogl C  et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep 18:2015-2029 (2017). PubMed: 28893864
    • Datta A  et al. Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibition. Cell Signal 40:91-98 (2017). PubMed: 28888686
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (RBL-2H3)
    Permeabilization
    Yes - 0.5% saponin
    Specification
    RBL-2H3
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 09 2021

    IHC - Wholemount abreview for Anti-EBP50/NHERF-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Tissue (FETAL MEMBRANE)
    Specification
    FETAL MEMBRANE
    Read More

    Dr. Ananth Kumar Kammala

    Verified customer

    提交于 Jan 29 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (AMNION EPITHELIAL CELLS)
    Permeabilization
    Yes - 0.3% TRITON-X-100
    Specification
    AMNION EPITHELIAL CELLS
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 29 2021

    Western blot abreview for Anti-EBP50/NHERF-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Fetal membrane cells)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    20 µg
    Treatment
    100ng of LPS
    Specification
    Fetal membrane cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Dr. Ananth Kumar Kammala

    Verified customer

    提交于 Jan 26 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (hepatocytes)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 17 2014

    Western blot abreview for Anti-EBP50/NHERF-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (Kidney tissue lysate)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Kidney tissue lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 10 2011

    Western blot abreview for Anti-EBP50 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2)
    Loading amount
    20 µg
    Specification
    HepG2
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 30 2008

    Western blot abreview for Anti-EBP50 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Loading amount
    25 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 30 2008

    Question

    ab3452 (EBP50 antibody) What epitope does this antibody recognize? I need to demonstrate to a reviewer that ab3452 is not crossreactive to tubulin.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2006

    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab3452 (EBP50 antibody). The epitope recognized by this product is amino acids 286-302 of the human EBP50 protein. This information has been added to the product datasheet. I hope this helps with the reviewers. Good luck with your manuscript submission. Please don't hesitate to contact us again if you have further questions.

    Read More

    Abcam Scientific Support

    回复于 Dec 29 2006

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 70213 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human cell lines HL60, Jurket and BJAB. whole cell lysates PRIMARY ANTIBODY ab3452: used at 1:1000, incubate at room temperature for 1 hour, wash 5 times and 15 minutes per wash ab9526: as above SECONDARY ANTIBODY ab3452: Goat anti rabbit IgG, 1:5000, [a competitor], incubated and washed per 1' ab9526: sheep anti mouse IgG, 1:5000, [a competitor], incubated and washed per 1' DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED ab3452: rabbit polyclonal anti-moesin [a competitor] ab9526: KPL1 which is a mouse monoclonal (IgG) to human PSGL-1 Both negative controls always gave us a band of the proper MW ANTIBODY STORAGE CONDITIONS The antibody was aliquoted and is stored at -20C. Working stock is thawed on ice and stored a 4C. SAMPLE PREPARATION 1% TX-100 lysis buffer with 4 different protease inhibitors. Samples were boiled for 2 minutes in SDS sample buffer AMOUNT OF PROTEIN LOADED WCL from 4 x 10^6 cells ELECTROPHORESIS/GEL CONDITIONS Non reduced, 7.5 % separating and 4 % stacking TRANSFER AND BLOCKING CONDITIONS Standard western transfer buffer, 1 hour, blocked with 2 % gelatin for 2 hours. MW markers transfered to Nitrocellulose (could visualize these as intense bands!) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES The comments are for both Abs I purchased from abcam. This is the second comment form I have filled out, I believe the first one was emailed on4/6/05. ab3452: gave us a very weak signal of the appropriate MW, no background bands were noted. ab9526: no appropriate signal and no background

    Read More

    Abcam community

    Verified customer

    Asked on Apr 08 2005

    Answer

    I'm sorry to hear you are having problems with ab3452 and ab9526 and you had to re-send us your protocol details, I appreciate you taking the time to let us know those. I would like to recommend incubating the primary antibodies overnight at 4C and to try more concentrated antibody (e.g 1:200-1:500). A good positive control for EBP50 is T84 cell lysate. Please do not hesitate to contact us again if you still have problems with the positive control and those recommendations,

    Read More

    Abcam Scientific Support

    回复于 Apr 11 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有