Anti-EBP50/NHERF-1抗体(ab3452)

Reviews (4)Q&A (2)References (33)

Key features and details

  • Rabbit polyclonal to EBP50/NHERF-1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-EBP50/NHERF-1抗体
    参阅全部 EBP50/NHERF-1 一抗

  • 描述

    兔多克隆抗体to EBP50/NHERF-1

  • 宿主

    Rabbit

  • 特异性

    Detects EBP50/NHERF-1 from human T84 and Calu3 cells as well as recombinant human protein.

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Dog, Human
    预测可用于: Rabbit, Cow, Cynomolgus monkey

  • 免疫原

    Recombinant fragment (His-tag) corresponding to Human EBP50/NHERF-1. All His-tag specific antibody has been removed.
    Sequence:

    RSASSDTSEELNSQDSP

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab3452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能

    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.

  • 组织特异性

    Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

  • 疾病相关

    Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).

  • 序列相似性

    Contains 2 PDZ (DHR) domains.

  • 翻译后修饰

    Phosphorylated on serine residues.

  • 细胞定位

    Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

图片

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole cell lysate prepared from human HepG2 cells

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 40 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)This image is courtesy of an anonymous abreview.
    All lanes : Anti-EBP50/NHERF-1 antibody (ab3452) at 1/1000 dilution

    All lanes : Whole tissue lysate prepared from mouse liver

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa


    Exposure time: 30 seconds

    See Abreview

  • Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)
    Western blot - Anti-EBP50/NHERF-1 antibody (ab3452)

    Western blot detection of recombinant Human EBP50/NHERF-1 using ab3452.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBP50/NHERF-1 antibody (ab3452)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse airway tissue sections labeling EBP50/NHERF-1 with ab3452.

文献 (33)

发表研究结果有使用 ab3452？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3452 被引用在 33 文献中.

  • Tsurudome Y  et al. Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function. Sci Rep 8:9072 (2018). PubMed: 29899468
  • Benhamouche-Trouillet S  et al. Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis. Development 145:N/A (2018). PubMed: 29712669
  • Kurtenbach S  et al. The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golfto Olfactory Cilia. Sci Rep 7:8409 (2017). PubMed: 28814779
  • Vogl C  et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep 18:2015-2029 (2017). PubMed: 28893864
  • Datta A  et al. Fibroblast-derived HGF drives acinar lung cancer cell polarization through integrin-dependent RhoA-ROCK1 inhibition. Cell Signal 40:91-98 (2017). PubMed: 28888686
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-EBP50/NHERF-1 antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jun 17 2014

Western blot abreview for Anti-EBP50/NHERF-1 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (Kidney tissue lysate)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Kidney tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Jan 10 2011

Western blot abreview for Anti-EBP50 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
20 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Oct 30 2008

Western blot abreview for Anti-EBP50 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
25 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (8)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Oct 30 2008

Question

ab3452 (EBP50 antibody) What epitope does this antibody recognize? I need to demonstrate to a reviewer that ab3452 is not crossreactive to tubulin.

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Answer

Thank you for your patience in this matter. I have received a reply from the originator of ab3452 (EBP50 antibody). The epitope recognized by this product is amino acids 286-302 of the human EBP50 protein. This information has been added to the product datasheet. I hope this helps with the reviewers. Good luck with your manuscript submission. Please don't hesitate to contact us again if you have further questions.

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Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 70213 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human cell lines HL60, Jurket and BJAB. whole cell lysates PRIMARY ANTIBODY ab3452: used at 1:1000, incubate at room temperature for 1 hour, wash 5 times and 15 minutes per wash ab9526: as above SECONDARY ANTIBODY ab3452: Goat anti rabbit IgG, 1:5000, [a competitor], incubated and washed per 1' ab9526: sheep anti mouse IgG, 1:5000, [a competitor], incubated and washed per 1' DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED ab3452: rabbit polyclonal anti-moesin [a competitor] ab9526: KPL1 which is a mouse monoclonal (IgG) to human PSGL-1 Both negative controls always gave us a band of the proper MW ANTIBODY STORAGE CONDITIONS The antibody was aliquoted and is stored at -20C. Working stock is thawed on ice and stored a 4C. SAMPLE PREPARATION 1% TX-100 lysis buffer with 4 different protease inhibitors. Samples were boiled for 2 minutes in SDS sample buffer AMOUNT OF PROTEIN LOADED WCL from 4 x 10^6 cells ELECTROPHORESIS/GEL CONDITIONS Non reduced, 7.5 % separating and 4 % stacking TRANSFER AND BLOCKING CONDITIONS Standard western transfer buffer, 1 hour, blocked with 2 % gelatin for 2 hours. MW markers transfered to Nitrocellulose (could visualize these as intense bands!) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES The comments are for both Abs I purchased from abcam. This is the second comment form I have filled out, I believe the first one was emailed on4/6/05. ab3452: gave us a very weak signal of the appropriate MW, no background bands were noted. ab9526: no appropriate signal and no background

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Answer

I'm sorry to hear you are having problems with ab3452 and ab9526 and you had to re-send us your protocol details, I appreciate you taking the time to let us know those. I would like to recommend incubating the primary antibodies overnight at 4C and to try more concentrated antibody (e.g 1:200-1:500). A good positive control for EBP50 is T84 cell lysate. Please do not hesitate to contact us again if you still have problems with the positive control and those recommendations,

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