Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic stem cell line (CCE))
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mouse embryonic stem cell line (CCE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% non-fat milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 47 kDa
Additional data
Additional Notes
Multiple unspecific bands were observed.
Abcam response
Thank you for sharing this data with us. ab32485 is batch tested in western blot on MEF1 cell lysates (please see image on datasheet). However, based on the data shown here this batch of ab32485 is not working well in western blot on mouse ES cell lysates, under these experimental conditions. We would welcome feedback from other researchers currently using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 27 2017