重组Anti-E2F4抗体[EPR8259] (ab150360)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8259] to E2F4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-E2F4抗体[EPR8259]
参阅全部 E2F4 一抗 -
描述
兔单克隆抗体[EPR8259] to E2F4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human E2F4 aa 250-350. The exact sequence is proprietary.
Database link: Q16254 -
阳性对照
- WB: HEK293T, K562, Raji, HeLa, and Jurkat (ab7899) cell lysates. IHC-P: Human kidney and lung adenocarcinoma tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8259 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150360于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein. -
组织特异性
Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the E2F/DP family. -
发展阶段
Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle. -
翻译后修饰
Differentially phosphorylated in vivo. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1874 Human
- Omim: 600659 Human
- SwissProt: Q16254 Human
- Unigene: 108371 Human
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别名
- E2F 4 antibody
- E2F transcription factor 4 antibody
- E2F transcription factor 4 p107/p130 binding antibody
see all
图片
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All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : E2F4 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab150360 observed at 60 kDa. Red - loading control ab8245 observed at 36 kDa.
ab150360 Anti-E2F4 antibody [EPR8259] was shown to specifically react with E2F4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266119 (knockout cell lysate ab257932) was used. Wild-type and E2F4 knockout samples were subjected to SDS-PAGE. ab150360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-E2F4 antibody [EPR8259] (ab150360) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling E2F4 with ab150360 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Thyroid gland papillary carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab150360 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab150360 被引用在 6 文献中.
- Zheng Q et al. Transcription factor E2F4 is an indicator of poor prognosis and is related to immune infiltration in hepatocellular carcinoma. J Cancer 12:1792-1803 (2021). PubMed: 33613768
- Su M et al. Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication. J Virol 95:e0018721 (2021). PubMed: 34037422
- Xu W et al. Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int 20:116 (2020). PubMed: 32308561
- Wang SA et al. USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy. Sci Rep 10:20870 (2020). PubMed: 33257797
- Dhanasekaran K et al. Unraveling the role of aurora A beyond centrosomes and spindle assembly: implications in muscle differentiation. FASEB J 33:219-230 (2019). PubMed: 29995440
- Li LL et al. JMJD2A contributes to breast cancer progression through transcriptional repression of the tumor suppressor ARHI. Breast Cancer Res 16:R56 (2014). PubMed: 24886710