Anti-E2F1抗体[KH95] (ab4070)

Reviews (1) Submit a question References (9)

Key features and details

  • Mouse monoclonal [KH95] to E2F1
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-E2F1抗体[KH95]
    参阅全部 E2F1 一抗

  • 描述

    小鼠单克隆抗体[KH95] to E2F1

  • 宿主

    Mouse

  • 特异性

    Protein of 60 kDa.

  • 经测试应用

    适用于: IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照

    • Tonsil and Breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab4070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

  • 序列相似性

    Belongs to the E2F/DP family.

  • 翻译后修饰

    Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Dmel\CG6376 antibody
    • Dmel_CG6376 antibody
    • drosE2F1 antibody
    • E(Sev-CycE)3A antibody
    • E(var)3-93E antibody
    • E2-promoter binding facto antibody
    • E2F 1 antibody
    • E2F transcription factor 1 antibody
    • E2F-1 antibody
    • E2f-PA antibody
    • E2f-PB antibody
    • E2f-PC antibody
    • E2F1 antibody
    • E2f1 E2F transcription factor 1 antibody
    • E2F1_HUMAN antibody
    • Evar(3)164 antibody
    • KIAA4009 antibody
    • l(3)07172 antibody
    • l(3)j3B1 antibody
    • l(3)j3C2 antibody
    • l(3)rM729 antibody
    • mKIAA4009 antibody
    • OTTHUMP00000030661 antibody
    • PBR3 antibody
    • PRB binding protein E2F 1 antibody
    • PRB-binding protein E2F-1 antibody
    • RBAP 1 antibody
    • RBAP-1 antibody
    • RBAP1 antibody
    • RBBP-3 antibody
    • RBBP3 antibody
    • RBP 3 antibody
    • RBP3 antibody
    • Retinoblastoma-associated protein 1 antibody
    • Retinoblastoma-binding protein 3 antibody
    • Transcription factor E2F1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [KH95] (ab4070)
    ab4070 staining human tonsil by IHC-P.

文献 (9)

发表研究结果有使用 ab4070？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4070 被引用在 9 文献中.

  • Zou Y  et al. ERK Inhibitor Enhances Everolimus Efficacy through the Attenuation of dNTP Pools in Renal Cell Carcinoma. Mol Ther Nucleic Acids 14:550-561 (2019). PubMed: 30771617
  • Tian T  et al. Long Noncoding RNA MPRL Promotes Mitochondrial Fission and Cisplatin Chemosensitivity via Disruption of Pre-miRNA Processing. Clin Cancer Res 25:3673-3688 (2019). PubMed: 30885939
  • Bervoets S  et al. Transcriptional dysregulation by a nucleus-localized aminoacyl-tRNA synthetase associated with Charcot-Marie-Tooth neuropathy. Nat Commun 10:5045 (2019). PubMed: 31695036
  • Kanakkanthara A  et al. ZC3H18 specifically binds and activates the BRCA1 promoter to facilitate homologous recombination in ovarian cancer. Nat Commun 10:4632 (2019). PubMed: 31604914
  • Rasmussen RD  et al. BRCA1-regulated RRM2 expression protects glioblastoma cells from endogenous replication stress and promotes tumorigenicity. Nat Commun 7:13398 (2016). ChIP . PubMed: 27845331
  • Tarangelo A  et al. Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression. Nat Commun 6:10028 (2015). IHC-P ; Mouse . PubMed: 26639898
  • Kopparapu PK  et al. MCPH1 maintains long-term epigenetic silencing of ANGPT2 in chronic lymphocytic leukemia. FEBS J 282:1939-52 (2015). PubMed: 25703238
  • Kim JE  et al. LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission. Cell Death Differ N/A:N/A (2014). WB . PubMed: 24561342
  • Millour J  et al. ATM and p53 Regulate FOXM1 Expression via E2F in Breast Cancer Epirubicin Treatment and Resistance. Mol Cancer Ther 10:1046-58 (2011). ChIP ; Human . PubMed: 21518729

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

ChIP abreview for Anti-E2F1 antibody [KH95] - ChIP Grade

 Poor
Abreviews
Application
ChIP
Sample
Mouse Cell lysate - whole cell (AML12 cells (normal mouse hepatocytes))
Specification
AML12 cells (normal mouse hepatocytes)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37% formaldehyde (final conc.
Detection step
Real-time PCR
Positive control
I used 10% of total cell lysate as a positive control
Negative control
I used non-antibody IP cell lysate as a negative control
Read More

Abcam user community

Verified customer

提交于 Aug 20 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com