Anti-E2 tag抗体[5E11] (ab977)
![Western blot - Anti-E2 tag antibody [5E11] (ab977)](https://www.abcam.cn/ps/products/0/ab977/Images/ab977_1.gif "Western blot - Anti-E2 tag antibody [5E11] (ab977)")
Key features and details
- Mouse monoclonal [5E11] to E2 tag
- Suitable for: IP, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-E2 tag抗体[5E11]
参阅全部 E2 tag 一抗 -
描述
小鼠单克隆抗体[5E11] to E2 tag -
宿主
Mouse -
经测试应用
适用于: IP, WB, ICC/IFmore details -
种属反应性
与反应: Species independent -
表位
SSTSSDFRDR (linear) -
常规说明
E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
5E11 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) |
Use at an assay dependent dilution.
|
| WB |
1/1000 - 1/5000. Incubate for 1 hour at room temperature. Background can be reduced by adding NaCl up to a concentration of 1.5M.
|
|
| ICC/IF | (1) |
1/1000. Incubate for 1 hour at room temperature.
|
| 说明 |
|---|
|
IP
Use at an assay dependent dilution. |
|
WB
1/1000 - 1/5000. Incubate for 1 hour at room temperature. Background can be reduced by adding NaCl up to a concentration of 1.5M. |
|
ICC/IF
1/1000. Incubate for 1 hour at room temperature. |
靶标
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相关性
The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein. -
别名
- E2 epitope tag antibody
- SSTSSDFRDR epitope tag antibody
- SSTSSDFRDR tag antibody
图片
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Western blot - Anti-E2 tag antibody [5E11] (ab977)Cos-7 cell lysates (1
µ g of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000). Cos-7 cell lysates (1µg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000). -
![Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977)](https://www.abcam.cn/ps/products/0/ab977/Images/ab977-3843-anti-e2-tag-antibody-5e11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977)This image is courtesy of an Abreview submitted by Dr Ashraf Mannanab977 at 1/200 dilution staining human Hela cells by ICC/IF. The HeLa cells were transfected with the fusion DNA construct pQM-NTag/A consisting of E2 tag fused in frame with gene specific cDNA. The cells were fixed with paraformaldehyde and blocked with serum prior to incubation with the antibody for 2 hours. A Cy3 conjugated sheep anti-mouse IgG antibody was used as the secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab977 被引用在 9 文献中.
- Murayama Y et al. Establishment of DNA-DNA Interactions by the Cohesin Ring. Cell 172:465-477.e15 (2018). WB . PubMed: 29358048
- Kaczmarek K et al. Overexpression of peroxisomal testis-specific 1 protein induces germ cell apoptosis and leads to infertility in male mice. Mol Biol Cell 22:1766-79 (2011). PubMed: 21460186
- Mannan AU et al. Spastin, the most commonly mutated protein in hereditary spastic paraplegia interacts with Reticulon 1 an endoplasmic reticulum protein. Neurogenetics 7:93-103 (2006). PubMed: 16602018
- Mannan AU et al. ZFYVE27 (SPG33), a novel spastin-binding protein, is mutated in hereditary spastic paraplegia. Am J Hum Genet 79:351-7 (2006). PubMed: 16826525
- Liewen H et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp Cell Res 306:24-34 (2005). PubMed: 15878329
- Koljak R et al. The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa. J Biol Chem 276:7033-40 (2001). PubMed: 11085996
- Kaldalu N et al. Functional domains of the TOL plasmid transcription factor XylS. J Bacteriol 182:1118-26 (2000). PubMed: 10648539
- Kaldalu N et al. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques 28:456-60, 462 (2000). PubMed: 10723557
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926