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Tags & Cell Markers Epitope Tags Other Tags E2 Tag

Anti-E2 tag抗体[5E11] (ab977)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (9)

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Western blot - Anti-E2 tag antibody [5E11] (ab977)
  • Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977)

Key features and details

  • Mouse monoclonal [5E11] to E2 tag
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-E2 tag抗体[5E11]
    参阅全部 E2 tag 一抗
  • 描述

    小鼠单克隆抗体[5E11] to E2 tag
  • 宿主

    Mouse
  • 经测试应用

    适用于: IP, WB, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 表位

    SSTSSDFRDR (linear)
  • 常规说明

    E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.4
    Constituent: 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    5E11
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • E2 Tag

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
    • Anti-E2 tag antibody [3F12] (ab978)
    • Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP (1)
Use at an assay dependent dilution.
WB
1/1000 - 1/5000. Incubate for 1 hour at room temperature. Background can be reduced by adding NaCl up to a concentration of 1.5M.
ICC/IF (1)
1/1000. Incubate for 1 hour at room temperature.
说明
IP
Use at an assay dependent dilution.
WB
1/1000 - 1/5000. Incubate for 1 hour at room temperature. Background can be reduced by adding NaCl up to a concentration of 1.5M.
ICC/IF
1/1000. Incubate for 1 hour at room temperature.

靶标

  • 相关性

    The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein.
  • 别名

    • E2 epitope tag antibody
    • SSTSSDFRDR epitope tag antibody
    • SSTSSDFRDR tag antibody

图片

  • Western blot - Anti-E2 tag antibody [5E11] (ab977)
    Western blot - Anti-E2 tag antibody [5E11] (ab977)
    Cos-7 cell lysates (1µg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000). Cos-7 cell lysates (1µg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000).
  • Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977)
    Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977)This image is courtesy of an Abreview submitted by Dr Ashraf Mannan
    ab977 at 1/200 dilution staining human Hela cells by ICC/IF. The HeLa cells were transfected with the fusion DNA construct pQM-NTag/A consisting of E2 tag fused in frame with gene specific cDNA. The cells were fixed with paraformaldehyde and blocked with serum prior to incubation with the antibody for 2 hours. A Cy3 conjugated sheep anti-mouse IgG antibody was used as the secondary.

    See Abreview

实验方案

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab977?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab977 被引用在 9 文献中.

  • Murayama Y  et al. Establishment of DNA-DNA Interactions by the Cohesin Ring. Cell 172:465-477.e15 (2018). WB . PubMed: 29358048
  • Kaczmarek K  et al. Overexpression of peroxisomal testis-specific 1 protein induces germ cell apoptosis and leads to infertility in male mice. Mol Biol Cell 22:1766-79 (2011). PubMed: 21460186
  • Mannan AU  et al. Spastin, the most commonly mutated protein in hereditary spastic paraplegia interacts with Reticulon 1 an endoplasmic reticulum protein. Neurogenetics 7:93-103 (2006). PubMed: 16602018
  • Mannan AU  et al. ZFYVE27 (SPG33), a novel spastin-binding protein, is mutated in hereditary spastic paraplegia. Am J Hum Genet 79:351-7 (2006). PubMed: 16826525
  • Liewen H  et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp Cell Res 306:24-34 (2005). PubMed: 15878329
  • Koljak R  et al. The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa. J Biol Chem 276:7033-40 (2001). PubMed: 11085996
  • Kaldalu N  et al. Functional domains of the TOL plasmid transcription factor XylS. J Bacteriol 182:1118-26 (2000). PubMed: 10648539
  • Kaldalu N  et al. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques 28:456-60, 462 (2000). PubMed: 10723557
  • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Immunoprecipitation abreview for Anti-E2 tag antibody [5E11]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
5 µg
Specification
HeLa
Immuno-precipitation step
Protein G
Read More

Dr. Ashraf Mannan

Verified customer

提交于 Dec 22 2006

Immunocytochemistry/ Immunofluorescence abreview for Anti-E2 tag antibody [5E11]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 30%
Read More

Dr. Ashraf Mannan

Verified customer

提交于 Nov 03 2006

Question

the vial our customer got as a replacement contains only 40 µl, not 100 µ of the antibody #AB977l. What now?

Read More

Abcam community

Verified customer

Asked on Dec 01 2004

Answer

My apologies for the inconvenience, I will arrange for a new vial to be sent to you in your next shipment. Thank you for your patience,

Read More

Abcam Scientific Support

回复于 Dec 01 2004

Question

BATCH NUMBER 9057 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal. The anti-E2 antibody lot # 9057 does not or only insufficiently detect the transiently in COS7, Alexander, Hep3B and HEK 293 cells expressed fusionprotein E2-p14.5 by westernblotting This same fusionprotein is detected on the same membrane by the same antibody ordered 6 month before Lot # 6260. SAMPLE HEK 293, COS7, Alexander, Hep3B Mammalian cells PRIMARY ANTIBODY Primary Antibody Details (Dilutions, Diluting Buffer) 1.AB over night 4?C or 2hrs RT, 3x10min PBS wash SECONDARY ANTIBODY 2.AB anti mouse (Biorad) 1:3000 1hr RT 3x10 min PBS wash, DETECTION METHOD chemiluminescence by ECL POSITIVE AND NEGATIVE CONTROLS USED old Lot #6260 of the same antibody ANTIBODY STORAGE CONDITIONS The vial was always kept ? 20 C? and never touched or opened before Nov .04. Aliquots of Lot # 6260 were kept in the same box/freezer and worked fine. SAMPLE PREPARATION Lysis buffer: 20mMTRIS ph 7.4 150mMNaCl 10% Glycerol 1% TritonX100 . Complete Protein Inhibitor (Roche) 1:3000 1:5000 1:10000 in 5% skim milk 3%BSA TRANSFER AND BLOCKING CONDITIONS 30 minutes preblocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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Abcam community

Verified customer

Asked on Nov 23 2004

Answer

I'm very sorry to hear you are having problems with ab977 lot 9057; we have not had any reported problems with this lot, it is possible the antibody has gone off when it was sent to you. I will arrange for a new vial to be sent to you as soon as possible with your shipment. We are currently out of stock of this antibody but should have some more in the next few days, my apologies for the delay. I would be very grateful if you could let me know if you are satisfied with the new lot. Thank you for your patience,

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Abcam Scientific Support

回复于 Nov 24 2004

Question

Is one of your anti-E2 tag antibodies better than the other for immunoprecipitation? How much have you used for immunoprecipitation experiments? Is one more sensitive for western blotting? If one is better for blotting and the other for immunoprecipitation, can you indicate anything about the magnitudes of the differences? Sincerely yours, John Dougherty

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Abcam community

Verified customer

Asked on Aug 14 2002

Answer

For immunoprecipitation and for affinity chromatography we recommend to use ab978 - during further elution milder conditions can be used. We have used ab978 beads for affinity purification of several different proteins in small quantities. For western blot it is better to use ab977 as this clone has higher affinity than ab978.

Read More

Abcam Scientific Support

回复于 Aug 16 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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