Key features and details
- Mouse monoclonal [3F12] to E2 tag
- Suitable for: WB, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
参阅全部 E2 tag 一抗
描述小鼠单克隆抗体[3F12] to E2 tag
经测试应用适用于: WB, IP, ICC/IFmore details
种属反应性与反应: Species independent
常规说明E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Concentration information loading...
纯度Protein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT) (dilute in 1% non-fat dry milk in TBST).
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性The E2Tag is a peptide which is derived from the Bovine Pappillomavirus type-1 transactivator protein E2 and consists of 10 amino acids(SSTSSDFRDR). The detection of E2-tagged proteins is based on the binding of mouse monoclonal antibodies specific to the tagged recombinant protein.
- E2 epitope tag antibody
- SSTSSDFRDR epitope tag antibody
- SSTSSDFRDR tag antibody
ab978 被引用在 7 文献中.
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- Auvinen E et al. Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein. Arch Virol 149:1745-59 (2004). PubMed: 15593417
- Ord D & Ord T Mouse NIPK interacts with ATF4 and affects its transcriptional activity. Exp Cell Res 286:308-20 (2003). PubMed: 12749859
- Koljak R et al. The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa. J Biol Chem 276:7033-40 (2001). PubMed: 11085996
- Kaldalu N et al. Functional domains of the TOL plasmid transcription factor XylS. J Bacteriol 182:1118-26 (2000). PubMed: 10648539
- Kaldalu N et al. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques 28:456-60, 462 (2000). PubMed: 10723557
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926