Key features and details
- Rabbit polyclonal to E tag
- Suitable for: ICC, ELISA, WB, ICC/IF, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run.|
|WB||1/1000 - 1/3000.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
相关性E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.
- E epitope tag antibody
- GAPVPYPDPLEPR epitope tag antibody
- GAPVPYPDPLEPR tag antibody
ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
ab3397 被引用在 6 文献中.
- Zhao L et al. Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1a. Sci Rep 9:11861 (2019). PubMed: 31413262
- Mont S et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . PubMed: 27118599
- Chavanayarn C et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel) 4:554-67 (2012). WB . PubMed: 22852068
- Leotoing L et al. A20-binding inhibitor of nuclear factor-kappaB (NF-kappaB)-2 (ABIN-2) is an activator of inhibitor of NF-kappaB (IkappaB) kinase alpha (IKKalpha)-mediated NF-kappaB transcriptional activity. J Biol Chem 286:32277-88 (2011). IP . PubMed: 21784860
- Chen TT et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
- Lampugnani MG et al. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol 174:593-604 (2006). PubMed: 16893970