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Tags & Cell Markers Epitope Tags Other Tags E tag

Anti-E tag抗体(ab3397)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (7)

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Immunocytochemistry - Anti-E tag antibody (ab3397)

    Key features and details

    • Rabbit polyclonal to E tag
    • Suitable for: ICC, ELISA, WB, ICC/IF, Flow Cyt
    • Reacts with: Species independent
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-E tag抗体
      参阅全部 E tag 一抗
    • 描述

      兔多克隆抗体to E tag
    • 宿主

      Rabbit
    • 经测试应用

      适用于: ICC, ELISA, WB, ICC/IF, Flow Cytmore details
    • 种属反应性

      与反应: Species independent
    • 免疫原

      Synthetic peptide: GAPVPYPDPLEPR conjugated to KLH.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.20
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Tags & Cell Markers
      • Epitope Tags
      • Other Tags
      • E tag

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3397 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    ICC (1)
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run.
    WB
    1/1000 - 1/3000.
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt (1)
    Use at an assay dependent concentration.

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

     

    说明
    ICC
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run.
    WB
    1/1000 - 1/3000.
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

     

    靶标

    • 相关性

      E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR.
    • 别名

      • E epitope tag antibody
      • GAPVPYPDPLEPR epitope tag antibody
      • GAPVPYPDPLEPR tag antibody

    图片

    • Immunocytochemistry - Anti-E tag antibody (ab3397)
      Immunocytochemistry - Anti-E tag antibody (ab3397)This image is a courtesy of Anonymous Abreview

      ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

      See Abreview

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (7)

    发表研究结果有使用 ab3397?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3397 被引用在 7 文献中.

    • Chekli Y  et al. Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Sci Rep 10:15791 (2020). PubMed: 32978420
    • Zhao L  et al. Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1a. Sci Rep 9:11861 (2019). PubMed: 31413262
    • Mont S  et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . PubMed: 27118599
    • Chavanayarn C  et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel) 4:554-67 (2012). WB . PubMed: 22852068
    • Leotoing L  et al. A20-binding inhibitor of nuclear factor-kappaB (NF-kappaB)-2 (ABIN-2) is an activator of inhibitor of NF-kappaB (IkappaB) kinase alpha (IKKalpha)-mediated NF-kappaB transcriptional activity. J Biol Chem 286:32277-88 (2011). IP . PubMed: 21784860
    • Chen TT  et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
    • Lampugnani MG  et al. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol 174:593-604 (2006). PubMed: 16893970

    客户评价及客户问答

    Show All 评价 Q&A
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    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-E tag antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Saccharomyces cerevisiae Cell (live yeast cells)
    Specification
    live yeast cells
    Preparation
    Cell harvesting/tissue preparation method: harvest cells then wash with 1XPBS
    Sample buffer: 1XPBS
    Fixation
    No fixation
    Permeabilization
    No
    Gating Strategy
    E-tag expression
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 10 2012

    Immunocytochemistry abreview for Anti-E tag antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HUVEC)
    Specification
    HUVEC
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% triton-X100
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 13 2009

    Question

    Has this product been tested for use in IHC-P?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 31 2005

    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

    Read More

    Abcam Scientific Support

    回复于 Mar 31 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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