Anti-E tag抗体(ab3397)
Key features and details
- Rabbit polyclonal to E tag
- Suitable for: ICC, ELISA, WB, ICC/IF, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-E tag抗体
参阅全部 E tag 一抗 -
描述
兔多克隆抗体to E tag -
宿主
Rabbit -
经测试应用
适用于: ICC, ELISA, WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide: GAPVPYPDPLEPR conjugated to KLH.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
|
| ELISA |
Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA).
A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction.
Appropriate specificity controls were run.
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| WB |
1/1000 - 1/3000.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| 说明 |
|---|
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ICC
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GAPVPYPDPLEPR-BSA). A 1/10,000 dilution of the antibody gave an O.D. = 1.0 in a 15 minute reaction. Appropriate specificity controls were run. |
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WB
1/1000 - 1/3000. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
靶标
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相关性
E tag is a peptide epitope tag, sequence GAPVPYPDPLEPR. -
别名
- E epitope tag antibody
- GAPVPYPDPLEPR epitope tag antibody
- GAPVPYPDPLEPR tag antibody
图片
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Immunocytochemistry - Anti-E tag antibody (ab3397)This image is a courtesy of Anonymous Abreview
ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
实验方案
文献 (7)
ab3397 被引用在 7 文献中.
- Chekli Y et al. Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Sci Rep 10:15791 (2020). PubMed: 32978420
- Zhao L et al. Intrabody against prolyl hydroxylase 2 promotes angiogenesis by stabilizing hypoxia-inducible factor-1a. Sci Rep 9:11861 (2019). PubMed: 31413262
- Mont S et al. Accumulation of isolevuglandin-modified protein in normal and fibrotic lung. Sci Rep 6:24919 (2016). IF . PubMed: 27118599
- Chavanayarn C et al. Humanized-single domain antibodies (VH/VHH) that bound specifically to Naja kaouthia phospholipase A2 and neutralized the enzymatic activity. Toxins (Basel) 4:554-67 (2012). WB . PubMed: 22852068
- Leotoing L et al. A20-binding inhibitor of nuclear factor-kappaB (NF-kappaB)-2 (ABIN-2) is an activator of inhibitor of NF-kappaB (IkappaB) kinase alpha (IKKalpha)-mediated NF-kappaB transcriptional activity. J Biol Chem 286:32277-88 (2011). IP . PubMed: 21784860
- Chen TT et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
- Lampugnani MG et al. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol 174:593-604 (2006). PubMed: 16893970
