E. coli Positive Control (Escherichia coli )全细胞裂解物(ab5395)

概述

  • 产品名称
    E. coli Positive Control (Escherichia coli )全细胞裂解物
  • 常规说明

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
    T7 HSV
    C-Myc
    VSV-g
    Glu-Glu
    V5
    E-tag
    FLAG (DDDDK)
    S-tag
    HA
    6x His

    GST

    Tag sequences: T7 = CMASMTGGQQMG
    HSV = QPELAPEDPEDC
    CMYC = EQKLISEEDLC
    VSV-g = YTDIEMNRLGK
    GluGlu = EYMPME
    V5 = GKPIPNPLLGLDST
    E-tag = GAPVPYPDPLEPR
    FLAG = DYKDDDDK
    HA = YPYDVPDYAC
    6xHIS = HHHHHH

     

    Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.

  • 经测试应用
    适用于: WBmore details

性能

应用

Our Abpromise guarantee covers the use of ab5395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

图片

  • All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl

文献

This product has been referenced in:
  • Májek P  et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). Read more (PubMed: 24278455) »
  • Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . Read more (PubMed: 21515633) »
See all 2 Publications for this product

客户评价及客户问答

1-3 of 3 Q&A

Answer

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très désolée pour le retard de cette réponse. J'espère que cette information vous est quand-même utile.

Aussi, est-ce que vous avez eu des bons résultats avec le nouveau tube de ab2431?

Encore une fois toutes mes excuses pour ce délais de réponse. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More

Question
Answer

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC HA = YPYDVPDYAC VSV-g = YTDIEMNRLGK GluGlu = EYMPME E-tag = GAPVPYPDPLEPR FLAG = DYKDDDDK V5 = TSTLPVDTTDWIEGETYK 6xHIS = HHHHHH I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册