E. coli Positive Control (Escherichia coli )全细胞裂解物(ab5395)

Q&A (3)Specific References (2)

概述

  • 产品名称
    E. coli Positive Control (Escherichia coli )全细胞裂解物
  • 常规说明

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
    T7 HSV
    C-Myc
    VSV-g
    Glu-Glu
    V5
    E-tag
    FLAG (DDDDK)
    S-tag
    HA
    6x His

    GST

    Tag sequences: T7 = CMASMTGGQQMG
    HSV = QPELAPEDPEDC
    CMYC = EQKLISEEDLC
    VSV-g = YTDIEMNRLGK
    GluGlu = EYMPME
    V5 = GKPIPNPLLGLDST
    E-tag = GAPVPYPDPLEPR
    FLAG = DYKDDDDK
    HA = YPYDVPDYAC
    6xHIS = HHHHHH

     

    Excellent as a loading control for Western blotting. Lysate expresses 11 different epitope tags.

  • 经测试应用
    适用于: WBmore details

性能

应用

Our Abpromise guarantee covers the use of ab5395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

图片

  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl

文献

This product has been referenced in:
  • Májek P  et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). Read more (PubMed: 24278455) »
  • Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . Read more (PubMed: 21515633) »

See all 2 Publications for this product

发表研究结果有使用 ab5395？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交问题

3 Q&A

10
Votes: Highest First

Hey,

Is the concentration of the tagged fusion protein known? If not, do you sell any myc-tagged fusion proteins where the concentration is known?

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesit...

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J’aurais besoin de savoir quelle est la souche d’E.Coli utilisée pour le lysat ab5395 (concernant le lysat ab2431 la souche utilisé est BL21).

Merci beaucoup pour votre retour

Cordialement

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très d...

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Do you have the sequence of each of the tags contained in the recombinant protein?

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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