E. coli Positive Control (Escherichia coli )全细胞裂解物(ab5395)
概述
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产品名称
E. coli Positive Control (Escherichia coli )全细胞裂解物 -
常规说明
This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:
GST
T7
HSV
C-Myc
VSV-g
Glu-Glu
V5
E-tag
FLAG (DDDDK)
S-tag
HA
6x His
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
1X Loading Buffer (1X SDS-PAGE Sample Buffer: 62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue) -
Concentration information loading... -
裂解物说明
Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.
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研究领域
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背景
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
Use 0.5 - 2.0 µl for 10 - 12% gels. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Use 0.5 - 2.0 µl for 10 - 12% gels. |
图片
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Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution
Lane 1 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
Lane 2 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
Lane 3 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
Lane 4 :E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl -
Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab5395 被引用在 3 文献中.
- Shokri L et al. A Comprehensive Drosophila melanogaster Transcription Factor Interactome. Cell Rep 27:955-970.e7 (2019). PubMed: 30995488
- Májek P et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). PubMed: 24278455
- Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . PubMed: 21515633