概述

  • 产品名称
    Dynasore,dynamin抑制剂
  • 描述
    Cell-permeable dynamin抑制剂
  • 生物学描述
    Cell-permeable dynamin inhibitor, that blocks endocytosis. Non-competitively inhibits dynamin1, dynamin2 and mitochondrial dynamin GTPase activity. Selective over other small GTPases.
  • 纯度
    > 99%
  • CAS编号
    304448-55-3
  • 化学结构
    Chemical Structure

性能

  • 化学名称
    3-Hydroxynaphthalene-2-carboxylic acid (3,4-dihydroxybenzylidene)hydrazide
  • 分子量
    322.31
  • 分子式
    C18H14N2O4
  • 存放说明
    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • 溶解度概述
    Soluble in DMSO to 100 mM
  • 处理

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES
    Oc3ccc(/C=N/NC(=O)c1cc2ccccc2cc1O)cc3O
  • 来源

    Synthetic

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab120192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

图片

  • ab66705 staining PAI1 in HeLa cells treated with dynasore (ab120192), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynasore, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dynasore) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Western blots of indicated proteins after knockdown with gene-specific siRNA against AP2M1 (ab120192) or non-targeting siRNA transfection control (NT).

    siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48h. The cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to ensure knockdown of the specific protein expression. The blots were also probed with β-actin specific antibody as the gel-loading control.

    Jens H et al. PLoS One. 2016; 11(10): e0164768. doi: 10.1371/journal.pone.0164768

文献

This product has been referenced in:
See all 24 Publications for this product

客户评价及客户问答

Answer

Thank you for your enquiry.

Please find information about the biological activity of these compounds below. We also have specific references listed on the datasheet that will provide more information. Reviewing the online literature I have also found additional information.

Dynasore ab120192
Cell permeable dynamin inhibitor, that blocks endocytosis. Non-competitively inhibits dynamin1, dynamin2 and mitochondrial dynamin GTPase activity. Selective over other small GTPases.

https://www.abcam.com/Dynasore-ab120192-references.html

Dynasore, a Dynamin Inhibitor, Inhibits Trypanosoma cruzi Entry into Peritoneal Macrophages

http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0007764


Dyngo-4a ab120689
Novel, highly potent dynamin inhibitor (IC50 values are 380 nM and 2.6 µM at dynamin I and II respectively). Dynasore (ab120192) analogue. Prevents botulin neurotoxin A (BoNTs) uptake and internalisation, and prevents SNAP25 cleavage. Inhibits BoNTs induced paralysis and delays botulism onset.

https://www.abcam.com/Dyngo-4a™-ab120689-references.html

Ascent Scientific Launch Novel Dynamin Inhibitor Dyngo-4a™ with Children's Medical Research Institute and University of Newcastle

http://www.ascentscientific.com/NewsItem.php?article=article011111


I would suggest browsing other online resources that may have additional specific information. I hope this information helps.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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