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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-Dynamin 3抗体(ab3458)

  • Datasheet
Reviews (2) Submit a question References (9)

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Western blot - Anti-Dynamin 3 antibody (ab3458)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 3 antibody (ab3458)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 3 antibody (ab3458)

Key features and details

  • Rabbit polyclonal to Dynamin 3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Dynamin 3抗体
    参阅全部 Dynamin 3 一抗
  • 描述

    兔多克隆抗体to Dynamin 3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Rat Dynamin 3 aa 623-639.
    Sequence:

    PDKSFTENDENGQAENF


    (Peptide available as ab4986)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab3458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. By Western blot, this antibody detects an ~100 kDa protein representing Dynamin 3 from HeLa cell lysate.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能

    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • 序列相似性

    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • 细胞定位

    Cytoplasm. Cytoplasm, cytoskeleton. Microtubule-associated.
  • Target information above from: UniProt accession Q9UQ16 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 26052 Human
    • Entrez Gene: 103967 Mouse
    • Entrez Gene: 171574 Rat
    • Omim: 611445 Human
    • SwissProt: Q9UQ16 Human
    • SwissProt: Q8BZ98 Mouse
    • SwissProt: Q08877 Rat
    • Unigene: 654775 Human
    • Unigene: 441620 Mouse
    • Unigene: 11191 Rat
    see all
  • 别名

    • testicular dynamin antibody
    • testicular antibody
    • DNM 3 antibody
    • DNM3 antibody
    • DNM3 protein antibody
    • Dyn3 antibody
    • DYN3_HUMAN antibody
    • Dyna III antibody
    • DynaIII antibody
    • dynamin 2 antibody
    • Dynamin antibody
    • Dynamin family member antibody
    • Dynamin testicular antibody
    • Dynamin-3 antibody
    • Dynamin3 antibody
    • EC 3.6.5.5 antibody
    • KIAA0820 antibody
    • MGC70433 antibody
    • T dynamin antibody
    • T-dynamin antibody
    see all

图片

  • Western blot - Anti-Dynamin 3 antibody (ab3458)
    Western blot - Anti-Dynamin 3 antibody (ab3458)

    Western blot of Dynamin 3 on rat liver extract using ab3458.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 3 antibody (ab3458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 3 antibody (ab3458)
    ab3458 (4µg/ml) staining Dynamin 3 in human brain cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the white matter region
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 3 antibody (ab3458)
    Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 3 antibody (ab3458)
    ICC/IF image of ab3458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (9)

    发表研究结果有使用 ab3458?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3458 被引用在 9 文献中.

    • Ding W  et al. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits. JCI Insight 3:N/A (2018). PubMed: 29563333
    • Li Y  et al. Transcriptomes of cochlear inner and outer hair cells from adult mice. Sci Data 5:180199 (2018). PubMed: 30277483
    • Gu C  et al. Dynamin 3 suppresses growth and induces apoptosis of hepatocellular carcinoma cells by activating inducible nitric oxide synthase production. Oncol Lett 13:4776-4784 (2017). PubMed: 28599479
    • Zhang Z  et al. DNM3 Attenuates Hepatocellular Carcinoma Growth by Activating P53. Med Sci Monit 22:197-205 (2016). WB ; Human . PubMed: 26784388
    • Hyndman KA  et al. Dynamin activates NO production in rat renal inner medullary collecting ducts via protein-protein interaction with NOS1. Am J Physiol Renal Physiol 301:F118-24 (2011). ICC/IF ; Rat . PubMed: 21490139
    • Wang W  et al. Distinct Functional Effects for Dynamin 3 During Megakaryocytopoiesis. Stem Cells Dev : (2011). ICC/IF . PubMed: 21671749
    • Xin X  et al. Kalirin12 interacts with dynamin. BMC Neurosci 10:61 (2009). WB ; Human, Rat . PubMed: 19534784
    • Mettlen M  et al. Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits. Mol Biol Cell 20:3251-60 (2009). WB ; Rat . PubMed: 19458185
    • Liu YW  et al. Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells. Mol Biol Cell 19:5347-59 (2008). WB, ICC/IF ; Mouse . PubMed: 18923138

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 3 antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (testis tissue)
    Specification
    testis tissue
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - cold acetone
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Dr. Wayne Vogl

    Verified customer

    提交于 Jan 29 2010

    Western blot abreview for Anti-Dynamin 3 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK-293T)
    Loading amount
    20 µg
    Specification
    HEK-293T
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 13 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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