Anti-Dynamin 3抗体(ab3458)
Key features and details
- Rabbit polyclonal to Dynamin 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Dynamin 3抗体
参阅全部 Dynamin 3 一抗 -
描述
兔多克隆抗体to Dynamin 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Dynamin 3 aa 623-639.
Sequence:PDKSFTENDENGQAENF
(Peptide available asab4986)
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed, and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Can be blocked with Dynamin 3 peptide (ab4986). By Western blot, this antibody detects an ~100 kDa protein representing Dynamin 3 from HeLa cell lysate. | |
| IHC-P | Use a concentration of 4 µg/ml. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. -
序列相似性
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
Contains 1 GED domain.
Contains 1 PH domain. -
细胞定位
Cytoplasm. Cytoplasm, cytoskeleton. Microtubule-associated. - Information by UniProt
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数据库链接
- Entrez Gene: 26052 Human
- Entrez Gene: 103967 Mouse
- Entrez Gene: 171574 Rat
- Omim: 611445 Human
- SwissProt: Q9UQ16 Human
- SwissProt: Q8BZ98 Mouse
- SwissProt: Q08877 Rat
- Unigene: 654775 Human
see all -
别名
- testicular dynamin antibody
- testicular antibody
- DNM 3 antibody
see all
图片
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Western blot - Anti-Dynamin 3 antibody (ab3458)Western blot of Dynamin 3 on rat liver extract using ab3458.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 3 antibody (ab3458)ab3458 (4µg/ml) staining Dynamin 3 in human brain cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartments within the white matter region
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 3 antibody (ab3458)ICC/IF image of ab3458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
文献 (9)
ab3458 被引用在 9 文献中.
- Ding W et al. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits. JCI Insight 3:N/A (2018). PubMed: 29563333
- Li Y et al. Transcriptomes of cochlear inner and outer hair cells from adult mice. Sci Data 5:180199 (2018). PubMed: 30277483
- Gu C et al. Dynamin 3 suppresses growth and induces apoptosis of hepatocellular carcinoma cells by activating inducible nitric oxide synthase production. Oncol Lett 13:4776-4784 (2017). PubMed: 28599479
- Zhang Z et al. DNM3 Attenuates Hepatocellular Carcinoma Growth by Activating P53. Med Sci Monit 22:197-205 (2016). WB ; Human . PubMed: 26784388
- Hyndman KA et al. Dynamin activates NO production in rat renal inner medullary collecting ducts via protein-protein interaction with NOS1. Am J Physiol Renal Physiol 301:F118-24 (2011). ICC/IF ; Rat . PubMed: 21490139
- Wang W et al. Distinct Functional Effects for Dynamin 3 During Megakaryocytopoiesis. Stem Cells Dev : (2011). ICC/IF . PubMed: 21671749
- Xin X et al. Kalirin12 interacts with dynamin. BMC Neurosci 10:61 (2009). WB ; Human, Rat . PubMed: 19534784
- Mettlen M et al. Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits. Mol Biol Cell 20:3251-60 (2009). WB ; Rat . PubMed: 19458185
- Liu YW et al. Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells. Mol Biol Cell 19:5347-59 (2008). WB, ICC/IF ; Mouse . PubMed: 18923138
