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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-Dynamin 2抗体(ab3457)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (35)

Product price, shipping and contact information

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Western blot - Anti-Dynamin 2 antibody (ab3457)
  • Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
  • Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)
  • Western blot - Anti-Dynamin 2 antibody (ab3457)

Key features and details

  • Rabbit polyclonal to Dynamin 2
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Non human primates
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Dynamin 2抗体
    参阅全部 Dynamin 2 一抗
  • 描述

    兔多克隆抗体to Dynamin 2
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Non human primates
    预测可用于: Cow
  • 免疫原

    Synthetic peptide corresponding to Human Dynamin 2 aa 760-779.
    Sequence:

    SPTPQRRPVSSIHPPGRPPA


    (Peptide available as ab4985)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Dynamin 2 protein (ab132947)

应用

Our Abpromise guarantee covers the use of ab3457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能

    Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
  • 组织特异性

    Ubiquitously expressed.
  • 疾病相关

    Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 序列相似性

    Belongs to the dynamin family.
    Contains 1 GED domain.
    Contains 1 PH domain.
  • 细胞定位

    Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells.
  • Target information above from: UniProt accession P50570 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1785 Human
    • Entrez Gene: 13430 Mouse
    • Entrez Gene: 25751 Rat
    • Omim: 602378 Human
    • SwissProt: P50570 Human
    • SwissProt: P39054 Mouse
    • SwissProt: P39052 Rat
    • Unigene: 211463 Human
    • Unigene: 433257 Mouse
    • Unigene: 11231 Rat
    see all
  • 别名

    • CMT2M antibody
    • CMTDI1 antibody
    • CMTDIB antibody
    • DI CMTB antibody
    • Dnm2 antibody
    • DYN II antibody
    • DYN2 antibody
    • DYN2_HUMAN antibody
    • Dynamin II antibody
    • Dynamin-2 antibody
    • Dynamin2 antibody
    • DynaminII antibody
    • DYNII antibody
    see all

图片

  • Western blot - Anti-Dynamin 2 antibody (ab3457)
    Western blot - Anti-Dynamin 2 antibody (ab3457)
    All lanes : Anti-Dynamin 2 antibody (ab3457)

    Lane 1 : SH-SY5Y whole cell lysate
    Lane 2 : PC-12
    Lane 3 : Neuro-2a
    Lane 4 : SK-N-AS
    Lane 5 : U- 87 MG
    Lane 6 : Rat Brain

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugated
  • Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)
    Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).

    The nuclear counterstain is DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)
    ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)
    Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)
    ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Dynamin 2 antibody (ab3457)
    Western blot - Anti-Dynamin 2 antibody (ab3457)

    Western blot detection of Dynamin 2 on mouse testes homogenate using ab3457.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (35)

    发表研究结果有使用 ab3457?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3457 被引用在 35 文献中.

    • Tian M  et al. The Expression of Dynamin 1, 2, and 3 in Human Hepatocellular Carcinoma and Patient Prognosis. Med Sci Monit 26:e923359 (2020). PubMed: 32573516
    • Wu Q  et al. Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells. J Neurosci 39:199-211 (2019). PubMed: 30381405
    • Chen SL  et al. Endophilin-A2-mediated endocytic pathway is critical for enterovirus 71 entry into caco-2 cells. Emerg Microbes Infect 8:773-786 (2019). PubMed: 31132962
    • Gong J  et al. Gulp1 controls Eph/ephrin trogocytosis and is important for cell rearrangements during development. J Cell Biol 218:3455-3471 (2019). PubMed: 31409653
    • Wong BS  et al. A Direct Podocalyxin-Dynamin-2 Interaction Regulates Cytoskeletal Dynamics to Promote Migration and Metastasis in Pancreatic Cancer Cells. Cancer Res 79:2878-2891 (2019). PubMed: 30975647
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neuron)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    neuron
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 13 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (hippocampal neuron)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    hippocampal neuron
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 13 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (COS-7)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    COS-7
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Ya Zhou

    Verified customer

    提交于 Oct 06 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dynamin 2 antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Primary alveolar type II epithelial cells)
    Specification
    Primary alveolar type II epithelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 11 2012

    Immunoprecipitation abreview for Anti-Dynamin 2 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (lymphoblasts)
    Total protein in input
    5000 µg
    Specification
    lymphoblasts
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 07 2006

    Question

    Your data sheet indicated this antibody was tested only for western blotting. Do you have any information at all for immunoprecipitations of dynamin-2?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 12 2003

    Answer

    Thank you for your enquiry. To our knowledge, Ab3457 has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Nov 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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