Anti-Dynamin 2抗体(ab3457)
Key features and details
- Rabbit polyclonal to Dynamin 2
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human, Non human primates
- Isotype: IgG
概述
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产品名称
Anti-Dynamin 2抗体
参阅全部 Dynamin 2 一抗 -
描述
兔多克隆抗体to Dynamin 2 -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human, Non human primates
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human Dynamin 2 aa 760-779.
Sequence:SPTPQRRPVSSIHPPGRPPA
(Peptide available asab4985)
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 µg/ml. |
靶标
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功能
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. -
组织特异性
Ubiquitously expressed. -
疾病相关
Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) [MIM:606482]. Charcot-Marie-Tooth disease (CMT) is a clinically and genetically heterogeneous disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. CMTDIB is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
序列相似性
Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells. - Information by UniProt
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数据库链接
- Entrez Gene: 1785 Human
- Entrez Gene: 13430 Mouse
- Entrez Gene: 25751 Rat
- Omim: 602378 Human
- SwissProt: P50570 Human
- SwissProt: P39054 Mouse
- SwissProt: P39052 Rat
- Unigene: 211463 Human
see all -
别名
- CMT2M antibody
- CMTDI1 antibody
- CMTDIB antibody
see all
图片
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Western blot - Anti-Dynamin 2 antibody (ab3457)All lanes : Anti-Dynamin 2 antibody (ab3457)
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : PC-12
Lane 3 : Neuro-2a
Lane 4 : SK-N-AS
Lane 5 : U- 87 MG
Lane 6 : Rat Brain
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugated -
Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HeLa cells labeling Dynamin 2 with ab3457 at 2 ug/ml followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at 1/2000 dilution (green).
The nuclear counterstain is DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 2 antibody (ab3457)ab3457 (1µg/ml) staining Dynamin 2 in human lung using an automated system (DAKO Autostainer Plus).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry - Anti-Dynamin 2 antibody (ab3457)ICC/IF image of ab3457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Dynamin 2 antibody (ab3457)Western blot detection of Dynamin 2 on mouse testes homogenate using ab3457.
实验方案
文献 (33)
ab3457 被引用在 33 文献中.
- Wu Q et al. Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells. J Neurosci 39:199-211 (2019). PubMed: 30381405
- Chen SL et al. Endophilin-A2-mediated endocytic pathway is critical for enterovirus 71 entry into caco-2 cells. Emerg Microbes Infect 8:773-786 (2019). PubMed: 31132962
- Gong J et al. Gulp1 controls Eph/ephrin trogocytosis and is important for cell rearrangements during development. J Cell Biol 218:3455-3471 (2019). PubMed: 31409653
- Craxton A et al. PAXX and its paralogs synergistically direct DNA polymerase ? activity in DNA repair. Nat Commun 9:3877 (2018). PubMed: 30250067
- Chernikova SB et al. Dynamin impacts homology-directed repair and breast cancer response to chemotherapy. J Clin Invest 128:5307-5321 (2018). PubMed: 30371505
