Anti-Dynamin 1抗体(ab3456)
Key features and details
- Rabbit polyclonal to Dynamin 1
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Dynamin 1抗体
参阅全部 Dynamin 1 一抗 -
描述
兔多克隆抗体to Dynamin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Dynamin 1 aa 633-647.
Sequence:KEKASETEENGSDSF
(Peptide available asab4984) -
阳性对照
- WB: Rat homogenate. ICC: SH-SY5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).
|
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| ICC |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa). |
|
ICC
Use at an assay dependent concentration. |
靶标
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功能
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. -
序列相似性
Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated. - Information by UniProt
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数据库链接
- Entrez Gene: 1759 Human
- Entrez Gene: 140694 Rat
- Omim: 602377 Human
- SwissProt: Q05193 Human
- SwissProt: P21575 Rat
- Unigene: 522413 Human
- Unigene: 10354 Rat
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别名
- B dynamin antibody
- D100 antibody
- DNM 1 antibody
see all
图片
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Western blot - Anti-Dynamin 1 antibody (ab3456)
Western blot of Dynamin 1 on rat brain homogenate using ab3456.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody (ab3456)Immunofluorescence analysis of Dynamin I was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3456 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
数据表及文件
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Datasheet download
文献 (10)
ab3456 被引用在 10 文献中.
- Ivanova D et al. CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. Cell Rep 30:2444-2459.e7 (2020). PubMed: 32075774
- Du JJ et al. Clathrin-independent but dynamin-dependent mechanisms mediate Ca2+-triggered endocytosis of the glutamate GluK2 receptor upon excitotoxicity. J Integr Neurosci 19:449-458 (2020). PubMed: 33070524
- Priya R et al. Activity Regulates Cell Death within Cortical Interneurons through a Calcineurin-Dependent Mechanism. Cell Rep 22:1695-1709 (2018). WB ; Mouse . PubMed: 29444424
- Patel A et al. RCAN1 links impaired neurotrophin trafficking to aberrant development of the sympathetic nervous system in Down syndrome. Nat Commun 6:10119 (2015). Mouse . PubMed: 26658127
- Zhang C et al. Screening and identification of dynamin-1 interacting proteins in rat brain synaptosomes. Brain Res 1543:17-27 (2014). IP ; Rat . PubMed: 24211660
- Tinelli E et al. Muscle-specific function of the centronuclear myopathy and Charcot-Marie-Tooth neuropathy-associated dynamin 2 is required for proper lipid metabolism, mitochondria, muscle fibers, neuromuscular junctions and peripheral nerves. Hum Mol Genet 22:4417-29 (2013). PubMed: 23813975
- Hyndman KA et al. Dynamin activates NO production in rat renal inner medullary collecting ducts via protein-protein interaction with NOS1. Am J Physiol Renal Physiol 301:F118-24 (2011). IHC-P ; Rat . PubMed: 21490139
- Xin X et al. Kalirin12 interacts with dynamin. BMC Neurosci 10:61 (2009). WB ; Human, Rat . PubMed: 19534784
- Chardonnet S et al. Large-scale study of phosphoproteins involved in long-term potentiation in the rat dentate gyrus in vivo. Eur J Neurosci 27:2985-98 (2008). WB ; Rat . PubMed: 18588538
- Liu YW et al. Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells. Mol Biol Cell 19:5347-59 (2008). PubMed: 18923138
