Anti-Dsk2抗体(ab4119)
Key features and details
- Rabbit polyclonal to Dsk2
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-Dsk2抗体 -
描述
兔多克隆抗体to Dsk2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein (S. cerevisiae). Fusion protein Dsk2 expressed in E. coli was purified and thrombin-digested full-length Dsk2 was used for antigen.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab4119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/10000. Detects a band of approximately 51 kDa. |
靶标
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相关性
Dsk2p is a member of a protein family that is characterized by possession of an N-terminal ubiquitin (Ub)-like (UbL) domain and a C-terminal Ub-associated (UBA) domain. In yeast, DSK2 was isolated as a suppressor of kar1, which is defective in spindle pole duplication. Although DSK2 is not an essential gene, yeast strains deleted for both DSK2 and RAD23 are temperature-sensitive for growth because of a block of spindle pole body duplication. Dsk2p and Rad23p seems to perform overlapping functions, although the sequence of Dsk2p is hardly similar to that of Rad23p overall except that they show 30% identity in their N-terminal UbL domains (residues 1-76) and that both contain UBA domains at their C terminus. Recent work on yeast Rad23p indicates that it is connected with the Ub-proteasome pathway; the N-terminal UbL domain interacts with the proteasome. There are genetic interactions between RAD23 and the polyubiquitin-binding subunit of the proteasome, RPN10, and Rad23p seems to inhibit polyubiquitin chain assembly in a cell-free assay system. It has recently been identified that Xenopus Dsk2-related protein, XDRP1, blocked Ub-dependent degradation of the cyclin A, and the human homologue of XDRP1, Plic1, is reported to form a link between the Ub machinery and the proteasome. Plic1 also is known as ubiquilin, independently isolated in a yeast two-hybrid screen by using presenilin as bait. -
细胞定位
Nuclear -
数据库链接
- SwissProt: P48510 Saccharomyces cerevisiae
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别名
- SHE4 antibody
- Ubiquitin domain containing protein DSK2 antibody
- YMR276W antibody
图片
数据表及文件
文献 (2)
ab4119 被引用在 2 文献中.
- Pfingsten JS et al. Mutually exclusive binding of telomerase RNA and DNA by Ku alters telomerase recruitment model. Cell 148:922-32 (2012). PubMed: 22365814
- Funakoshi M et al. Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc Natl Acad Sci U S A 99:745-50 (2002). PubMed: 11805328