存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab43847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 222 kDa.|
功能Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
组织特异性Primarily expressed in brain.
序列相似性Contains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
结构域Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
翻译后修饰Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
细胞定位Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
- Information by UniProt
- GenBank: AF260530 Drosophila melanogaster
- CHD2 42 antibody
- CHD2 52 antibody
- CHD2 antibody
Anti-Dscam antibody (ab43847) at 1 µg/ml + Drosophila S2 Cells at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 222 kDa
Observed band size: 235 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute