概述

  • 产品名称
    Anti-ds DNA抗体[11B6]
    参阅全部 ds DNA 一抗
  • 描述
    小鼠单克隆抗体[11B6] to ds DNA
  • 宿主
    Mouse
  • 特异性
    In ELISA : This antibody shows 100% reactivity to calf dsDNA and 5% to calf ssDNA. It does not react with RNA.
  • 经测试应用
    适用于: ELISAmore details
  • 常规说明
    This antibody can only be used on highly purified dsDNA that has been stripped of chromatin. Histones can be removed by using an incubation step with proteinase K. We also recommend that you dilute no more than 1:10 - also try a 1:5 dilution.

性能

  • 形式
    Lyophilised:Reconstitute with 1ml sterile water.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    11B6
  • 骨髓瘤
    unknown
  • 同种型
    IgM
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5 - 1/10. We recommend that you dilute no more than 1/10 - also try a 1/5 dilution.

靶标

  • 相关性
    dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
  • 细胞定位
    Nuclear
  • 别名
    • Double stranded deoxyribonucleic acid antibody
    • Double stranded DNA antibody
    • dsDNA antibody

文献

ab418 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-10 of 14 Abreviews or Q&A

Answer

Thank you for contacting us.

Please check the following primary antibody datasheets

https://www.abcam.com/ds-DNA-antibody-HYB331-01-ab27156.html

https://www.abcam.com/ds-DNA-antibody-11B6-ab418.html

The compatible secondary antibodies are;

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG2a-heavy-chain-PE-Texas-Red-ab51410.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. Please check the datasheet for anti DNA antibodies ab27156; https://www.abcam.com/ds-DNA-antibody-HYB331-01-ab27156.html ab418; https://www.abcam.com/ds-DNA-antibody-11B6-ab418.html Please check the compatible secondary’s also ab8516 ab8517 ab8518 ab9175 ab97007 ab97008 ab97010 We unfortunately do not have anti RNA antibodies. Please select antibodies against RNA associated proteins.  I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Question
Answer

Thank you for your call this week and for your patience while I have been in touch with the lab regarding ab418. This antibody has not been specifically tested against plasmids, however it should recognize double-stranded DNA free of chromatin as described on the datasheet. Please let me know if you have any further questions about this and I will be happy to help.

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Answer

Thank you for your enquiry. I can tell you that the testing of this antibody was performed by immunohistochemistry using acetone fixed Hep2 and Hela cells. Unfortunately I do not have further details as to where this antibody has been applied on LR white resin embedded thin sections. The epitope of this antibody is dsDNA. Unfortunately I do not have specific information as to what within the DNA molecule it recognises other than that it recognises ds as opposed to ss DNA and requires a minimum of 10 bases (one complete helical turn) to recognise its epitope. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

This antibody will work on DNA measurement (by Elisa method).

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Answer

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). We will update the on-line datasheet of this product as soon as get more data.

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Question
Answer

Because it is tissue culture supernatant the concentration is difficult to determine, but we estimate it is approx. 0.1mg/ml.

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Answer

10 base pairs

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Answer

This antibody has been used in immunhistochemistry and ELISA applications. We do not have any reports of it being used for purification of double stranded DNA, though it may be suitable for this purpose.

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1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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