重组Anti-DR1抗体[EPR13122] - BSA and Azide free (ab250168)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13122] to DR1 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-DR1抗体[EPR13122] - BSA and Azide free
参阅全部 DR1 一抗 -
描述
兔单克隆抗体[EPR13122] to DR1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment within Human DR1. The exact sequence is proprietary.
Database link: Q01658 -
常规说明
ab250168 is the carrier-free version of ab180164.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13122 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab250168于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
序列相似性
Belongs to the NC2 beta/DR1 family.
Contains 1 histone-fold domain. -
翻译后修饰
Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1810 Human
- Entrez Gene: 13486 Mouse
- Entrez Gene: 289881 Rat
- Omim: 601482 Human
- SwissProt: Q01658 Human
- SwissProt: Q91WV0 Mouse
- SwissProt: Q5XI68 Rat
- Unigene: 348418 Human
see all -
别名
- Down regulator of transcription 1 antibody
- Down regulator of transcription 1 TBP binding antibody
- Down-regulator of transcription 1 antibody
see all
图片
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All lanes : Anti-DR1 antibody [EPR13122] (ab180164) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDaThis data was developed using ab180164, the same antibody clone in a different buffer formulation.
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This data was developed using ab180164, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling DR1 using ab180164 at 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab180164, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling DR1 using ab180164 at 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab180164, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) pulling down DR1 using ab180164 at 1/10 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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