DoubleStain IHC试剂盒: R&Rt on人/小鼠组织(Green/HRP & AP/Red) (ab183285)
概述
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产品名称
DoubleStain IHC试剂盒: R&Rt on人/小鼠组织(Green/HRP & AP/Red) -
检测类型
Polymer Multiplex -
产品概述
Abcam's Doublestain IHC Kit (ab183285) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples. This kit has been tested on paraffin embedded tissue, however, it can also be used to stain frozen specimens or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistostaining, allowing for the detection of two distinct antigens in a single tissue. Abcam's Doublestain IHC Kit (ab183285) supplies the user with two polymer enzyme conjugates: Rat HRP Polymer (minimal cross reaction to mouse) and Rabbit AP Polymer with two distinct substrates/chromogens, Emerald and Permanent Red. Emerald chromogen reacts with the Rat HRP polymer conjugate to produce a green color. Permanent Red reacts with the Rabbit AP polymer to produce a red color. When two proteins are co-localized a blue/ purple color will develop depending on which antigen is stronger. If only the rat antigen is present only the Emerald chromogen will be present and if the rabbit antigen is present only the Permanent Red chromogen will be present. Abcam's Doublestain IHC Kit (ab183285) is a non-biotin system avoiding the extra steps involved in blocking non-specific binding due to endogenous biotin.
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经测试应用
适用于: IHC-Fr, IHC-Pmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 36 ml 60 ml 6 ml 120 ml Emerald Chromogen 2 x 18ml 1 x 60ml 1 x 6ml 1 x 120ml Non-aqueous Mounting Medium 4 x 9ml 0 x 0ml 1 x 6ml 0 x 0ml Permanent Red Activator (5x) 1 x 7.2ml 1 x 24ml 1 x 3ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 360µl 1 x 1.2ml 1 x 150µl 1 x 1.2ml Permanent Red Substrate 2 x 18ml 1 x 120ml 1 x 15ml 1 x 120ml Rabbit AP Polymer 1 x 18ml 1 x 60ml 1 x 6ml 1 x 60ml Rat HRP Polymer 1 x 18ml 1 x 60ml 1 x 6ml 1 x 60ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab183285 被引用在 2 文献中.
- Leal AS et al. Retinoid X receptor agonist LG100268 modulates the immune microenvironment in preclinical breast cancer models. NPJ Breast Cancer 5:39 (2019). PubMed: 31700995
- Bathini P et al. Progressive signaling changes in the olfactory nerve of patients with Alzheimer's disease. Neurobiol Aging 76:80-95 (2019). PubMed: 30708185