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Neuroscience Neurotransmitter Biogenic Amines Dopamine

Anti-Dopamine Transporter抗体[hDAT-NT] (ab5990)

  • Datasheet
  • SDS
Reviews (4)Q&A (7)References (15)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)

    Key features and details

    • Rat monoclonal [hDAT-NT] to Dopamine Transporter
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Dopamine Transporter抗体[hDAT-NT]
      参阅全部 Dopamine Transporter 一抗
    • 描述

      大鼠单克隆抗体[hDAT-NT] to Dopamine Transporter
    • 宿主

      Rat
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Mouse, Human
      预测可用于: Rat
    • 免疫原

      Fusion protein corresponding to Dopamine Transporter aa 1-66 (N terminal).

    • 阳性对照

      • IHC-P: Mouse brain tissue.
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Constituents: PBS, 0.81% Sodium chloride, 0.16% Sodium phosphate, 0.02% Potassium chloride, 0.04% Potassium phosphate
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      hDAT-NT
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmitter
      • Biogenic Amines
      • Dopamine
      • Neuroscience
      • Neurotransmitter
      • Transporters
      • Dopamine

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG - Isotype Control (ab37361)
    • Recombinant Protein

      • Recombinant Human Dopamine Transporter protein (ab112352)

    应用

    Our Abpromise guarantee covers the use of ab5990 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    靶标

    • 功能

      Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
    • 疾病相关

      Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
    • 序列相似性

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession Q01959 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 6531 Human
      • Entrez Gene: 13162 Mouse
      • Entrez Gene: 24898 Rat
      • Omim: 126455 Human
      • SwissProt: Q01959 Human
      • SwissProt: Q61327 Mouse
      • SwissProt: P23977 Rat
      • Unigene: 406 Human
      • Unigene: 41993 Mouse
      • Unigene: 10093 Rat
      see all
    • 别名

      • DA transporter antibody
      • DAT 1 antibody
      • DAT antibody
      • DAT1 antibody
      • Dopamine transporter 1 antibody
      • Dopamine transporter antibody
      • PKDYS antibody
      • SC6A3_HUMAN antibody
      • SLC6A3 antibody
      • Sodium dependent dopamine transporter antibody
      • Sodium-dependent dopamine transporter antibody
      • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
      • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
      • Solute carrier family 6 member 3 antibody
      • Variable number tandem repeat (VNTR) antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)Carl Hobbs (Kings College, London, United Kingdom)

      ab5990 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A Biotin-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (15)

    发表研究结果有使用 ab5990?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5990 被引用在 15 文献中.

    • Playne R  et al. Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors. J Stem Cells Regen Med 14:34-44 (2018). PubMed: 30018471
    • Dijkstra AA  et al. Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function. Cereb Cortex 28:131-144 (2018). PubMed: 27913432
    • Maeder CI  et al. The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival. Cell 174:1436-1449.e20 (2018). PubMed: 30146163
    • Postupna N  et al. Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE e4. Am J Pathol 187:884-895 (2017). PubMed: 28212814
    • Alam G  et al. Single low doses of MPTP decrease tyrosine hydroxylase expression in the absence of overt neuron loss. Neurotoxicology 60:99-106 (2017). PubMed: 28377118
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    ELISA abreview for Anti-Dopamine Transporter antibody [hDAT-NT]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Recombinant protein (Recombinant Human Dopamine Transporter protein (ab)
    Specification
    Recombinant Human Dopamine Transporter protein (ab
    Blocking step
    MSD block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Type
    Direct
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 16 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dopamine Transporter antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    提交于 May 16 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Dopamine Transporter antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Karine Thibault

    Verified customer

    提交于 Aug 19 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dopamine Transporter antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (rat dopaminergic neuron)
    Specification
    rat dopaminergic neuron
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    saponin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 05 2013

    Question


    A user would like a testing discount code:

    Product: Anti-FOX3/NeuN antibody [1B7] (ab104224)
    Test: on human cell by IHC-Fr
    Product: Anti-Dopamine Transporter antibody (ab5990)
    Test: on human cell by ICC/IF

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2012

    Answer

    Thank you for contacting us.

    ab104224
    DISCOUNT CODE: xxxxxxxx
    Expiration date: xxxxxxxxxx

    I am very pleased to hear that your customer would like to accept our offer and test ab104224 in IHC-Fr. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    ab5990
    DISCOUNT CODE: xxxxxxxxx
    Expiration date: xxxxxxxxxxx

    I am very pleased to hear that your customer would like to accept our offer and test ab5990 in IF. To redeem this offer, please submit an Abreview for IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Abcam Scientific Support

    回复于 Nov 08 2012

    Question

    Pasted you can find the questionnaire you sent us. We look forward to hearing from you soon.
    1) Abcam product code ab
    ab5990

    2) Abcam order reference number or product batch number

    3) Description of the problem
    The antibody does not detect the predicted band for DAT (74 KDa)

    4) Sample preparation:
    Type of sample (whole cell lysates, fraction, recombinant protein…): Brain tissue (N. Accumbens) homogenate
    Lysis buffer
    Protease inhibitors: Roche
    Phosphatase inhibitors: Calbiochem
    Reducing agent: 2-mercaptoethanol
    Boiling for ≥5 min: yes
    Protein loaded ug/lane or cells/lane: 40-60 ug protein/lane
    Positive control
    Negative control

    5) Percentage of gel: 10%
    Type of membrane: PVDF
    Protein transfer verified: Yes
    Blocking agent and concentration: 1% BSA in TBS-Tween 1,5%
    Blocking time: > 1h
    Blocking temperature: Room Temperature

    6) Primary antibody (If more than one was used, describe in “additional notes”) :
    Concentration or dilution: 1:500 – 1:300
    Diluent buffer: TBS-Tween 1,5%
    Incubation time: 20 h aprox.
    Incubation temperature: 1-2 h Room Temperature - 4º C overnight

    7) Secondary antibody:
    Species: goat anti-rat
    Reacts against: rat IgG
    Concentration or dilution: 1:5000 – 1:2000
    Diluent buffer: TBS-Tween 1,5%
    Incubation time: 1h
    Incubation temperature: Room Temperature
    Fluorochrome or enzyme conjugate: HRP

    8) Washing after primary and secondary antibodies:
    Buffer: TBS-Tween 1,5%
    Number of washes: 3 (10 min each wash)

    9) Detection method: Chemifluorescence
    10) How many times have you run this staining?
    Do you obtain the same results every time? Yes
    What steps have you altered to try and optimize the use of this antibody?
    We have changed the ug Protein/lane, we have increased primary and secondary antibodies concentrations.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 31 2012

    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I would like to reassure you that ab5990 is tested and covered by our 6 month guarantee for use in WB and mouse, rat and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

    Reviewing this case, I would like to offer some suggestions to help optimise the results from ab5990.

    1.) I suggest to decrease the boiling temperature to 65C and to prolong the heating time up to 10 min. Your target is a multi-pass membrane protein, and these proteins have the unfortunate tendency to aggregate during boiling periods with high temperatures and beta- mercaptoethanol due to their hydrophobic nature.

    2.) I also recommend to reduce the amount of Tween to 0.1%. Too much tween will lead to reduced binding of the antibody since the tween is acting as detergent.

    I would also appreciate if you can confirm some further details:

    Is the secondary antibody working with other primary antibodies?

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

    Read More

    Abcam Scientific Support

    回复于 Jul 31 2012

    Question

    Dear abcam Team,

    i would like to know how long the Rat monoclonal to dopamine Treansporter (ab5990) is stable at -20oC.

    Are there any problems with the concentration/binding to the tube or... expected?

    Thanks a lot!

    Read More

    Abcam community

    Verified customer

    Asked on Apr 03 2012

    Answer

    Thank you for your enquiry,.

    We recommend that antibodies should be stable for at least 1 year at the recommended storage temperature. This can be much longer for frozen antibodies. However, please noteour Abpromise guarantee is provided for 6 months after purchase.

    With regards to concentration and binding to the tube when freezing, the best way to avoid this is to ensure aliquots are no less than 10 ul and ensure the lid on the tube is pressed well closed. Smaller volumes can evaporate and dry out easily, altering concentration as well as product quality.

    I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Apr 03 2012

    Question

    Can you please send me information about Dopamine Transporter antibodies that can be used in WB on rat samples?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2011

    Answer

    Thank you for contacting us.  We have three Dopamine Transporter antibodies that will be guaranteed for use in WB on rat samples: ab5981, ab5990, and ab18548.  Please find their datasheets attached.  I would be happy to help you further if you have any additional questions about our products. 

    Read More

    Abcam Scientific Support

    回复于 Nov 29 2011

    Question

    Please inform us the availability of blocking peptides for ab5990, ab5981 and ab9528. If we could buy them, could you tell us the protocol for the blocking peptides?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2005

    Answer

    Thank you for your enquiry. I regret to inform you that blocking peptides are not available for these 3 antibodies. If you have any additional questions, please don't hesitate to contact us again.

    Read More

    Abcam Scientific Support

    回复于 Sep 08 2005

    Question

    I AM INTERESTED IN PURCHASING A DOPAMINE ANTIBODY FOR ICC WORK ON HELMINTHS (PARASITIC FLATWORMS). CAN YOU ADVISE ME ON A SUITABLE ONE TO PURCHASE? IF AT ALL POSSIBLE COULE YOU SUPPLY A SMALL SAMPLE OF THE ANTIBODY TO ENSURE REACTIVITY AS I WILL BE INTENDING TO PURCHASE THE SUCCESSFUL ANTIBODY ON A REGULAR BASIS AND THEY ARE EXPENSIVE?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 02 2005

    Answer

    Thank you for your enquiry. Are looking for a dopamine transporter antibody or a dopamine antibody? You indicated ab5990 - Rat monoclonal to Dopamine Transporter - which is suitable for use in ICC but has not yet been tested for cross-reactivity with species other than human and rat. Ab5981 is another suggestion; it is a rat monoclonal [hDAT-LOOP] to Dopamine Transporter and works in ICC, although has not yet been tested for cross-reactivity with species other than human and rat. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image). If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Sep 02 2005

    1-10 of 11 Abreviews or Q&A

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