Anti-Dopamine Transporter抗体[hDAT-NT] (ab5990)

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ab104224
DISCOUNT CODE: xxxxxxxx
Expiration date: xxxxxxxxxx

I am very pleased to hear that your customer would like to accept our offer and test ab104224 in IHC-Fr. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


ab5990
DISCOUNT CODE: xxxxxxxxx
Expiration date: xxxxxxxxxxx

I am very pleased to hear that your customer would like to accept our offer and test ab5990 in IF. To redeem this offer, please submit an Abreview for IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab5990 is tested and covered by our 6 month guarantee for use in WB and mouse, rat and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab5990.

1.) I suggest to decrease the boiling temperature to 65C and to prolong the heating time up to 10 min. Your target is a multi-pass membrane protein, and these proteins have the unfortunate tendency to aggregate during boiling periods with high temperatures and beta- mercaptoethanol due to their hydrophobic nature.

2.) I also recommend to reduce the amount of Tween to 0.1%. Too much tween will lead to reduced binding of the antibody since the tween is acting as detergent.

I would also appreciate if you can confirm some further details:

Is the secondary antibody working with other primary antibodies?

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for your enquiry,.

We recommend that antibodies should be stable for at least 1 year at the recommended storage temperature. This can be much longer for frozen antibodies. However, please noteour Abpromise guarantee is provided for 6 months after purchase.

With regards to concentration and binding to the tube when freezing, the best way to avoid this is to ensure aliquots are no less than 10 ul and ensure the lid on the tube is pressed well closed. Smaller volumes can evaporate and dry out easily, altering concentration as well as product quality.

I hope this information will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Thank you for contacting us.  We have three Dopamine Transporter antibodies that will be guaranteed for use in WB on rat samples: ab5981, ab5990, and ab18548.  Please find their datasheets attached.  I would be happy to help you further if you have any additional questions about our products. 

Thank you for your enquiry. I regret to inform you that blocking peptides are not available for these 3 antibodies. If you have any additional questions, please don't hesitate to contact us again.

Thank you for your enquiry. Are looking for a dopamine transporter antibody or a dopamine antibody? You indicated ab5990 - Rat monoclonal to Dopamine Transporter - which is suitable for use in ICC but has not yet been tested for cross-reactivity with species other than human and rat. Ab5981 is another suggestion; it is a rat monoclonal [hDAT-LOOP] to Dopamine Transporter and works in ICC, although has not yet been tested for cross-reactivity with species other than human and rat. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image). If you have any additional questions, please contact us again.