Anti-Dopamine Transporter抗体[hDAT-NT] (ab5990)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)](https://www.abcam.cn/ps/products/5/ab5990/Images/ab5990-264046-anti-dopamine-transporter-antibody-hdat-nt-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)")
Key features and details
- Rat monoclonal [hDAT-NT] to Dopamine Transporter
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Dopamine Transporter抗体[hDAT-NT]
参阅全部 Dopamine Transporter 一抗 -
描述
大鼠单克隆抗体[hDAT-NT] to Dopamine Transporter -
宿主
Rat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse
预测可用于: Rat
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免疫原
Fusion protein corresponding to Dopamine Transporter aa 1-66 (N terminal).
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阳性对照
- IHC-P: Mouse brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituents: PBS, 0.81% Sodium chloride, 0.16% Sodium phosphate, 0.02% Potassium chloride, 0.04% Potassium phosphate -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
hDAT-NT -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
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功能
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. -
疾病相关
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. -
序列相似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13162 Mouse
- Entrez Gene: 24898 Rat
- SwissProt: Q61327 Mouse
- SwissProt: P23977 Rat
- Unigene: 41993 Mouse
- Unigene: 10093 Rat
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别名
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [hDAT-NT] (ab5990)Carl Hobbs (Kings College, London, United Kingdom)
ab5990 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/1000) for two hours at room temperature. A Biotin-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab5990 被引用在 15 文献中.
- Playne R et al. Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors. J Stem Cells Regen Med 14:34-44 (2018). PubMed: 30018471
- Dijkstra AA et al. Von Economo Neurons and Fork Cells: A Neurochemical Signature Linked to Monoaminergic Function. Cereb Cortex 28:131-144 (2018). PubMed: 27913432
- Maeder CI et al. The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival. Cell 174:1436-1449.e20 (2018). PubMed: 30146163
- Postupna N et al. Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE e4. Am J Pathol 187:884-895 (2017). PubMed: 28212814
- Alam G et al. Single low doses of MPTP decrease tyrosine hydroxylase expression in the absence of overt neuron loss. Neurotoxicology 60:99-106 (2017). PubMed: 28377118