Anti-Dopamine Receptor D3/DRD3抗体(ab42114)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D3/DRD3
- Suitable for: ELISA, Dot blot, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Dopamine Receptor D3/DRD3抗体
参阅全部 Dopamine Receptor D3/DRD3 一抗 -
描述
兔多克隆抗体to Dopamine Receptor D3/DRD3 -
宿主
Rabbit -
经测试应用
适用于: ELISA, Dot blot, ICC/IF, IP, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Dopamine Receptor D3/DRD3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42114于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells. |
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WB | (1) |
1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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说明 |
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ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. 2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells. |
WB
1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
靶标
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功能
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. -
组织特异性
Brain. -
疾病相关
Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma). -
细胞定位
Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. - Information by UniProt
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数据库链接
- Entrez Gene: 1814 Human
- Entrez Gene: 13490 Mouse
- Entrez Gene: 29238 Rat
- Omim: 126451 Human
- SwissProt: P35462 Human
- SwissProt: P30728 Mouse
- SwissProt: P19020 Rat
- Unigene: 121478 Human
see all -
别名
- 3dopamine receptor antibody
- D antibody
- D(3) dopamine receptor antibody
see all
图片
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Anti-Dopamine Receptor D3/DRD3 antibody (ab42114) at 1/750 dilution + Dopamine Receptor D3 Positive Control (rat)
Predicted band size: 50 kDa
Observed band size: 50 kDa -
ICC/IF image of ab42114 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (14)
ab42114 被引用在 14 文献中.
- Williford SE et al. Novel dopamine receptor 3 antagonists inhibit the growth of primary and temozolomide resistant glioblastoma cells. PLoS One 16:e0250649 (2021). PubMed: 33945569
- Kisling A et al. Loss of Function in Dopamine D3 Receptor Attenuates Left Ventricular Cardiac Fibroblast Migration and Proliferation in vitro. Front Cardiovasc Med 8:732282 (2021). PubMed: 34708087
- Reyes-Resina I et al. Expression of Melatonin and Dopamine D3 Receptor Heteromers in Eye Ciliary Body Epithelial Cells and Negative Correlation with Ocular Hypertension. Cells 9:N/A (2020). PubMed: 31936298
- Huang J et al. Involvement of dopamine D3 receptor and dopamine transporter in methamphetamine-induced behavioral sensitization in tree shrews. Brain Behav 10:e01533 (2020). PubMed: 31943832
- Xu K et al. Validation of the targeted metabolomic pathway in the hippocampus and comparative analysis with the prefrontal cortex of social defeat model mice. J Neurochem 149:799-810 (2019). PubMed: 30520040