重组Anti-Dopamine Receptor D1抗体[EP1560Y] (ab81296)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Mouse, Rat, Guinea pig, Human
Related conjugates and formulations
概述
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产品名称
Anti-Dopamine Receptor D1抗体[EP1560Y]
参阅全部 Dopamine Receptor D1 一抗 -
描述
兔单克隆抗体[EP1560Y] to Dopamine Receptor D1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat, Guinea pig, Human -
免疫原
Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21728 -
阳性对照
- WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1560Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81296于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
靶标
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功能
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
组织特异性
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
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数据库链接
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 2624 Human
see all -
别名
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
图片
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All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Guinea pig brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab81296 被引用在 16 文献中.
- Wu M et al. Electroacupuncture Alleviates Anxiety-Like Behaviors Induced by Chronic Neuropathic Pain via Regulating Different Dopamine Receptors of the Basolateral Amygdala. Mol Neurobiol 59:5299-5311 (2022). PubMed: 35696012
- Qian X et al. Dopamine Pathway Mediated by DRD5 Facilitates Tumor Growth via Enhancing Warburg Effect in Esophageal Cancer. Front Oncol 11:655861 (2021). PubMed: 33898321
- Tavares G et al. Dopamine D2 receptor agonist, bromocriptine, remodels adipose tissue dopaminergic signalling and upregulates catabolic pathways, improving metabolic profile in type 2 diabetes. Mol Metab N/A:101241 (2021). PubMed: 33933677
- Liu G et al. Inactivation of Cys674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase. Br J Pharmacol 177:1793-1805 (2020). PubMed: 31758704
- Lin CC et al. Early life social experience affects adulthood fear extinction deficit and associated dopamine profile abnormalities in a rat model of PTSD. Prog Neuropsychopharmacol Biol Psychiatry 101:109914 (2020). PubMed: 32165120