为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Neuroscience Neurotransmitter Biogenic Amines Dopamine

Anti-Dopamine抗体(ab6427)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (9)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry - Anti-Dopamine antibody (ab6427)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine antibody (ab6427)

Key features and details

  • Rabbit polyclonal to Dopamine
  • Suitable for: ICC, ICC/IF, ELISA
  • Reacts with: Species independent
  • Isotype: IgG

你可能会感兴趣

二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-Dopamine抗体
    参阅全部 Dopamine 一抗
  • 描述

    兔多克隆抗体to Dopamine
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, ICC/IF, ELISAmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule by a Glutaraldehyde linker.

  • 常规说明

    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • Primary antibody说明

    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Ghrelin (rat), Endogenous GHS agonist (ab120231)

应用

Our Abpromise guarantee covers the use of ab6427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. Glutharaldehyde perfusion fixation (see recommended protocol).
ELISA 1/5000.

靶标

  • 相关性

    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • 细胞定位

    Secreted
  • 别名

    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all

图片

  • Immunocytochemistry - Anti-Dopamine antibody (ab6427)
    Immunocytochemistry - Anti-Dopamine antibody (ab6427)This image is courtesy of an anonymous Abreview
    ab6427 at 1/5000 staining human stem cells (differentiated to neurons) by ICC. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 2 hours. A PE conjugated goatanti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine antibody (ab6427)
    Immunocytochemistry/ Immunofluorescence - Anti-Dopamine antibody (ab6427)
    ab6427 staining dopamine in PC12 cells treated with ghrelin (rat) (ab120231), by ICC/IF. Decrease in dopamine expression correlates with increased concentration of ghrelin, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120231 (ghrelin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6427 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

实验方案

  • Immunohisto and cytochemistry
  • Recommended protocol for ELISA with ab6427

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (9)

    发表研究结果有使用 ab6427?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6427 被引用在 9 文献中.

    • Brazdis RM  et al. Demonstration of brain region-specific neuronal vulnerability in human iPSC-based model of familial Parkinson's disease. Hum Mol Genet 29:1180-1191 (2020). PubMed: 32160287
    • Ohlendorf R  et al. Target-responsive vasoactive probes for ultrasensitive molecular imaging. Nat Commun 11:2399 (2020). PubMed: 32404879
    • Fink J  et al. Development of a Competition-Binding Assay to Determine Binding Affinity of Molecules to Neuromelanin via Fluorescence Spectroscopy. Biomolecules 9:N/A (2019). PubMed: 31072013
    • Taylor-Whiteley TR  et al. Recapitulating Parkinson's disease pathology in a three-dimensional human neural cell culture model. Dis Model Mech 12:N/A (2019). PubMed: 30926586
    • Auletta A  et al. Tyrosine hydroxylase immunolabeling reveals the distribution of catecholaminergic neurons in the central nervous systems of the spiders Hogna lenta (Araneae: Lycosidae) and Phidippus regius (Araneae: Salticidae). J Comp Neurol N/A:N/A (2019). PubMed: 31343075
    • Chabrat A  et al. Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons. Stem Cells Int 2019:2945435 (2019). PubMed: 31236114
    • Banks DA  et al. MK-STYX Alters the Morphology of Primary Neurons, and Outgrowths in MK-STYX Overexpressing PC-12 Cells Develop a Neuronal Phenotype. Front Mol Biosci 4:76 (2017). ICC/IF ; Rat . PubMed: 29250526
    • Wu SF  et al. Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis. Sci Rep 5:12247 (2015). PubMed: 26179416
    • Forati E  et al. Neurotransmitter Specific, Cellular-Resolution Functional Brain Mapping Using Receptor Coated Nanoparticles: Assessment of the Possibility. PLoS One 10:e0145852 (2015). PubMed: 26717196

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    I am interested in an anti-dopamine antibody and I see that you have ab6427. Does this antibody work on formalin fixed tissue? Does it work on rat tissue?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 10 2012

    Answer

    Thank you for contacting us.

    ab6427 is not guaranteed for use in immunohistochemistry. However, ab1001 (https://www.abcam.com/ab1001) is guaranteed to work in immunohistochemistry and gave good results when fixed with formalin/PFA in paraffin-embedded sections.

    Regarding your question about whether this antibody works on rat tissue, because the immunogen of ab1001 and ab6427 is a Chemical / Small Molecule, the species in which this antibody is used is irrelevant. Therefore both these antibodies should work on rat tissue.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Dec 10 2012

    Immunocytochemistry abreview for Anti-Dopamine antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (stem cells differentiated to neurons)
    Specification
    stem cells differentiated to neurons
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2
    Read More

    Abcam user community

    Verified customer

    提交于 May 29 2007

    Question

    Thanks for your quick response. I did check the web site but the info is not specific enough for me to understand the detailed procedures on how the DA is captured and if it needs to be separated from cell/tissue lysates before assaying and what's the sensitivity of the detection if it's quantitative.

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2005

    Answer

    We have additional information about capturing the DA as follows: After discussion with our research team, we think that the better way is to bind DA on your tissue via glutaraldehyde (0.1 to 1%). - Double bounds are reduced by adding of sodium borohydrure. - Tissue has to be wash for eliminate borohydrure excess. - You have also to make a competition scale between antibody and different concentration of the antigen DA-G-BSA. Had this medium on the tissue. - After revelation by a labelled anti-rabbit antibody, you finally have to estimate the competition rate between DA linked on tissue and soluble DA-G-BSA conjugate.

    Read More

    Abcam Scientific Support

    回复于 May 25 2005

    Question

    Is gluteraldehyde fixation required because the immunogen was glutaldehye-linked to BSA? We would like to try this on sections from animals already perfused with 4% paraformaldehyde.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 11 2004

    Answer

    Thank you for your enquiry. For Immunohistological studies with dopamine antibodies, fixation with glutaraldehyde is required. Please find below a protocol. Perfusion protocol for adult male Sprague-Dawley (weight around 0.5 kg) 1- The animals can be deeply anaesthetized for example with uerethane (0.1-1.5g/kg, intraperitoneal). 2- Heparinized and perfused via the ascending aorta with 100 ml of cold physiologic saline (0.9% NaCl) and with the following fixative solution: a. 300 ml of cold 4% paraformaldehyde and 2% glutaraldehyde in 0.1M Phosphate buffer (PB), pH 7.2 ( in two minutes). b. 600 ml of cold 4% paraformaldehyde and 2% glutaraldehyde in 0.1M PB, pH 7.2 ( in ten minutes). c. Dissect out the brains and place in a solution of 4% paraformaldehyde in 0.1M PB, pH 7.2, at 4°C for twelve to sixteen hours. d. Before brain will be cut on a freezing microtome, we must include it in growing concentrations of sucrose (5 at 30 %). If you have any further questions then please do not hesitate to get back in touch with us.

    Read More

    Abcam Scientific Support

    回复于 Nov 15 2004

    Question

    What type of pump is used and what company sells it?

    Read More

    Abcam community

    Verified customer

    Asked on May 29 2001

    Answer

    The pump that was used is a peristaltic pump with a flow of 150-300ml/minute. GILSON supply this pump type.

    Read More

    Abcam Scientific Support

    回复于 May 31 2001

    Question

    I would like to know if this antibody works well on floating slices of rat perfused with 4% paraformaldehyde and without glutaraldehyde. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 05 2001

    Answer

    This antibody is known to work with 3-5% glutaraldehyde perfusion. Protocol is as follows - Perfusion : perfuse intracardially through the aorta using a pump with the following solutions : solution A (30ml) : 200-300ml/min solution B (500ml) : 200-300ml/min Solution A : cacodylate 0.1M, sodium metabisulfite 10g/l, pH = 6.2 Solution B : cacodylate 0.1M, sodium metabisulfite 10g/l and glutaraldehyde 3-5% pH = 7.5 Post fixation : 15 to 30 min in solution B, then 4 soft washes in Tris 0.05M with sodium metabisulfite 8.5g/l, pH 7.5 (solution C). Tissue sectioning : Cryostat or vibratome sections can be used. Reduction step : Sections are reduced with the solution C containing sodium borohydride (0.1M) for 10 min. Then, the sections are washed 4 times with solution C without sodium borohydride. Application of anti-Dopamine antibodies : The final dilution is 1/2,000 to 1/5,000 in solution C containing triton X100 0.1%, plus 2% of non-specific serum. A dozen sections can be incubated with 2ml of antibody solution overnight at 4°C. Then the sections are washed 3 times (10 min) with solution C. N.B. : Antibodies may be used at a higher dilution. The customer should explore the antibody dilution to reduce the possibility of high background. Note that a substitution in the buffer system as used in our protocol may change the background and the antibody recognition. PAP procedure : Secondary antibody : Sections are incubated with 1/100 dilution of goat anti-rabbit in solution C for 3 hours at 20°C or 1 hour at 37°C. Then washed 3 times (10 min) with solution C. PAP : Sections are incubated with 1/1,000 dilution of rabbit peroxidase anti-peroxidase complex in solution C for 1 hour at 37°C. Then wash 3 times (10 min) with solution C. Detection : Antibody-antigen complexes are revealed using diaminobenzidine (25mg/100ml) (or other chromogen) dissolved in Tris 0.05M and filtrated; 0.05% of H2O2 is added. The sections are incubated for 10 min at 20°C. Reaction is stopped by transfering sections in 5ml of Tris 0.05M.

    Read More

    Abcam Scientific Support

    回复于 Apr 05 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    ?ICP?14046373?-1

    ????? 31011502007413?

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有