Application
Immunohistochemistry (Frozen sections)
I used this antibody to detect sheep anti tyrosine hydroxylase (TH) labelling of splenic nerves in mouse spleen sections along with rat anti-CD35 labelling of follicular dendritic cells (FDC) and rabbit anti PrP labelling of prion protein. It was diluted 1/200 for use on frozen sections that were fixed in acetone and blocked with serum for 30 mins at room temperature. I chose this antibody as I found it difficult to find secondary's that were anti-sheep. I was concerned that the FITC might bleach during confocal microscopy however I was able to scan the triple labelling without any bleaching occurring.
Image is of red CD35+ FDC, blue PrP and green TH+ nerves detected using donkey anti-sheep_FITC
Image is of red CD35+ FDC, blue PrP and green TH+ nerves detected using donkey anti-sheep_FITC
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Laura McCulloch
Verified customer
提交于 Oct 23 2013