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驴抗羊IgG H&L (DyLight® 550) (ab96940)

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Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)

    Key features and details

    • Donkey Anti-Sheep IgG H&L (DyLight® 550)
    • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: IHC-P, ICC/IF, Flow Cyt

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 650 FITC Gold 12nm HRP Texas Red ® TRITC Unconjugated

    概述

    • 产品名称

      驴抗羊IgG H&L (DyLight® 550)
      参阅全部 IgG 二抗

    • 宿主

      Donkey

    • 靶标种属

      Sheep

    • 特异性

      By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

    • 经测试应用

      适用于: IHC-P, ICC/IF, Flow Cytmore details

    • 偶联物

      DyLight® 550. Ex: 562nm, Em: 576nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: 0.2% BSA, PBS
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 常规说明

      DyLight® 550 replaces DyLight® 549.

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab96940于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.
    说明
    IHC-P
    1/50 - 1/500.

    DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. For more information please refer to DyLight® 550.
    ICC/IF
    1/50 - 1/500.
    Flow Cyt
    1/50 - 1/200.

    图片

    • Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)
      Donkey Anti-Sheep IgG H&L (DyLight® 550) (ab96940)
      Emission spectra of DyLight® fluorochromes available in our catalog.
      Line colors represent the approximate visible colors of the wavelength maxima.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab96940？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab96940 被引用在 1 文献中.

    • Mikedis MM & Downs KM Widespread but tissue-specific patterns of interferon-induced transmembrane protein 3 (IFITM3, FRAGILIS, MIL-1) in the mouse gastrula. Gene Expr Patterns 13:225-39 (2013). PubMed: 23639725

    客户评价及客户问答

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    Question

    Hi,

    I have reviewed this profile. I might not clarify clearly what I need. I need to have the spectrum of the dye as you may know the excitation spectrum may cover a certain range not only at a certain point. I am doing multiple staining of different proteins so the overlap of either excitation or emission affect the result a lot. Can you please give me more details of your dye? (such as dye light). Thanks a lot!

    all the best

    Read More
    Answer

    Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

    I ma sorry there was a misunderstanding.

    We do have information on the spectra of DyLight® fluorochromes on our homepage:

    https://www.abcam.com/index.html?pageconfig=resource&rid=12934

    The spectra can be reviewed on the bottom of the page. Unfortunately, we do not have a spectra viewer where multiple dyes can be combined and tested for overlapping. I am sorry if this causes any inconvenience.

    DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. Maybe it is worth checking their website for a spectra viewer also.

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

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