驴抗羊IgG H&L (Alexa Fluor® 594) (ab150180)
Key features and details
- Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594)
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗羊IgG H&L (Alexa Fluor® 594)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Sheep -
经测试应用
适用于: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Alexa Fluor® 594. Ex: 590nm, Em: 617nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
常规说明
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150180于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
1/200 - 1/1000.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
1/2000.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
1/2000. |
图片
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ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 2µg/ml) overnight at +4°C. Ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150180 Alexa Fluor® 594 donkey anti-sheep IgG (H+L) (orange) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab150180 被引用在 8 文献中.
- Liu X et al. A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses. NPJ Vaccines 8:132 (2023). PubMed: 37679361
- Wang Y et al. Identification of the cross-strand chimeric RNAs generated by fusions of bi-directional transcripts. Nat Commun 12:4645 (2021). PubMed: 34330918
- Dombrowski ME et al. Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress. Neurospine 17:69-76 (2020). PubMed: 32252156
- Larriva-Sahd J et al. On the existence of mechanoreceptors within the neurovascular unit of the mammalian brain. Brain Struct Funct 224:2247-2267 (2019). PubMed: 31190162
- Besse HC et al. Tumor Drug Distribution after Local Drug Delivery by Hyperthermia, In Vivo. Cancers (Basel) 11:N/A (2019). PubMed: 31600958
- Baur K et al. c-Fos marking of identified midbrain neurons coactive after nicotine administration in-vivo. J Comp Neurol 526:2019-2031 (2018). PubMed: 29888787
- Egunsola AT et al. Loss of DDRGK1 modulates SOX9 ubiquitination in spondyloepimetaphyseal dysplasia. J Clin Invest 127:1475-1484 (2017). PubMed: 28263186
- Morimoto K et al. Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice. Endocrinology 157:1071-81 (2016). PubMed: 26789236