驴抗大鼠IgG H&L (DyLight® 488)预吸附二抗(ab102260)
Key features and details
- Donkey Anti-Rat IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC, IHC-P
Related conjugates and formulations
概述
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产品名称
驴抗大鼠IgG H&L (DyLight® 488)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: Flow Cyt, ICC, IHC-Pmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
偶联物
DyLight® 488. Ex: 493nm, Em: 518nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102260于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IF |
1/50 - 1/500.
|
|
Flow Cyt |
1/50 - 1/200.
|
|
ICC |
1/50 - 1/500.
|
|
IHC-P |
1/50 - 1/500.
|
说明 |
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IF
1/50 - 1/500. |
Flow Cyt
1/50 - 1/200. |
ICC
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab102260 被引用在 4 文献中.
- Flores I et al. Myeloid cell-mediated targeting of LIF to dystrophic muscle causes transient increases in muscle fiber lesions by disrupting the recruitment and dispersion of macrophages in muscle. Hum Mol Genet 31:189-206 (2021). PubMed: 34392367
- Welc SS et al. Differential Effects of Myeloid Cell PPARd and IL-10 in Regulating Macrophage Recruitment, Phenotype, and Regeneration following Acute Muscle Injury. J Immunol 205:1664-1677 (2020). PubMed: 32817369
- Welc SS et al. Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy. Nat Commun 10:2788 (2019). PubMed: 31243277
- Cottle DL et al. c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis. Cell Rep 3:427-41 (2013). PubMed: 23403291