驴抗兔IgG H&L (Biotin)预吸附二抗(ab97083)
Key features and details
- Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, IHC-P, ICC/IF, ELISA
概述
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产品名称
驴抗兔IgG H&L (Biotin)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
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经测试应用
适用于: WB, IHC-P, ICC/IF, ELISAmore details -
最小交叉反应
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details -
免疫原
Other Immunogen Type corresponding to Goat IgG.
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97083于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/200000. (Chemiluminescent)
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IHC-P |
1/250 - 1/2500.
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ICC/IF |
1/100 - 1/500.
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ELISA |
1/10000 - 1/200000. (Primary ELISA)
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说明 |
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WB
1/10000 - 1/200000. (Chemiluminescent) |
IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. (Primary ELISA) |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab97083 被引用在 4 文献中.
- Ma P et al. RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission. Sci Adv 8:eabq4736 (2022). PubMed: 36179027
- Lagedal R et al. Impaired Antibody Response Is Associated with Histone-Release, Organ Dysfunction and Mortality in Critically Ill COVID-19 Patients. J Clin Med 11:N/A (2022). PubMed: 35743491
- Huckriede J et al. Evolution of NETosis markers and DAMPs have prognostic value in critically ill COVID-19 patients. Sci Rep 11:15701 (2021). PubMed: 34344929
- Huckriede J et al. Histone H3 Cleavage in Severe COVID-19 ICU Patients. Front Cell Infect Microbiol 11:694186 (2021). PubMed: 34568088