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Immunology Immunoglobulins Heavy Chain IgG

驴抗兔IgG H&L (Alexa Fluor® 647) (ab150075)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (237)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
  • Flow Cytometry - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
  • ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

Key features and details

  • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647)
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-Fr

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm Gold 6nm HRP IRDye® 680RD Texas Red ® TRITC Unconjugated Unconjugated

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概述

  • 产品名称

    驴抗兔IgG H&L (Alexa Fluor® 647)
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Rabbit
  • 经测试应用

    适用于: ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-Frmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rabbit
    • IgG
    • Fluorophore
    • Alexa Fluor® 647

相关产品

  • Alternative Versions

    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150074)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150076)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 568) (ab175470)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) (ab175651)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 750) (ab175731)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) (ab175772)
    • Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175780)
    • Donkey Anti-Rabbit IgG H&L (ab182020)
    • Donkey Anti-Rabbit IgG H&L (HRP) (ab205722)
    • Donkey Anti-Rabbit IgG H&L (Biotin) (ab207999)
  • Related Products

    • Anti-CD3 antibody [SP7] (ab16669)
    • Normal Donkey Serum (ab7475)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab150075于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.

IHC-Fr (2)
Use at an assay dependent concentration.
说明
ICC/IF
1/200 - 1/1000.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
1/2000 - 1/4000.

ab199093 - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control to complement this secondary antibody.

IHC-Fr
Use at an assay dependent concentration.

图片

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

    ICC/IF image of ab6046 stained HeLa cells. The cells were 100% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (red) was ab150075 Alexa Fluor® 647 donkey anti-rabbit IgG (H+L) used at 1µg/ml for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    Flow Cytometry - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

    Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Donkey anti-rabbit IgG H&L (Alexa Fluor® 647) (ab150075) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.

  • ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    ELISA - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

    Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50 µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.

    This data was developed using the unconjugated antibody (ab182020).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)This image is courtesy of an anonymous Abreview.

    ab62050 staining prostaglandin E synthase in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100, and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 20 hours at 4°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG H&L (ab150075) (1/200) was used as the secondary antibody.

    See Abreview

  • Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
    Alexa Fluor® - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (237)

发表研究结果有使用 ab150075?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab150075 被引用在 237 文献中.

  • Choi HJ  et al. Single-cell RNA sequencing of mitotic-arrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens. J Anim Sci Biotechnol 13:64 (2022). IHC-P ; Chicken . PubMed: 35659766
  • Liu X  et al. Hypoxic bone marrow mesenchymal cell-extracellular vesicles containing miR-328-3p promote lung cancer progression via the NF2-mediated Hippo axis. J Cell Mol Med 25:96-109 (2021). PubMed: 33219752
  • Vukušic K  et al. Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Dev Cell 56:1253-1267.e10 (2021). PubMed: 33910056
  • Huang J  et al. Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis. Bioact Mater 6:770-782 (2021). PubMed: 33024898
  • Choi DH  et al. 3D cell culture using a clinostat reproduces microgravity-induced skin changes. NPJ Microgravity 7:20 (2021). PubMed: 34075058
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

Excellent secondary antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
This antibody was used to visualise the endothelial cells (CD31+) in human spleen by indirect immunfluorescence.
The anti-rabbit antibody gives specific and strong signals on cryo preserved tissue sections.
Concentration 1:500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 04 2022

Glycogen synthase kinase-3 inhibition attenuates fibroblast activation and development of fibrosis following renal ischemia-reperfusion in mice.

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
I tried many different product to get secondary antibody for immunofluorescence staining better and more clear but failed then I bought abcam ab150075 - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) in kidney fibroblast cells, as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best secondary antibody to detect immunofluorescence during in vitro studies
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Shailendra Singh

Verified customer

提交于 Aug 17 2018

very reliable antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry
my go-to secondary for IHC. I use a 1:500 dilution in 1XPBS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 11 2016

Microglia in hippocampus of brain organotypic slice culture

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Good staining of microglia in green in brain slice counter stained with DAPI in blue. Scale bar 20um, magnification x40 oil.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 01 2016

Sox2 in the E10.5 neural tube.

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
E10.5 mouse embryo section. Samples were fixed with PFA and stained without antigen retrieval.
Primary: Sox2 ab92494 (1:300).
Secondary: Alexa Fluor 647 ab150075 (1:1000).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 27 2014

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