Application
Immunocytochemistry/ Immunofluorescence
Following fixation in 4% PFA, the cells were assessed for Sox2 expression using 1:100 dilution of the primary antibody (ab97959) in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using donkey polyclonal rabbit IgG Alexa 488 (ab150073) at 1:500. The results show that nuclear Sox-2 (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here). All nuclei were counterstained using DAPI (blue).
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提交于 Sep 02 2013