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驴抗小鼠IgG H&L (Texas Red ®)预吸附二抗(ab7059)

Submit a review Q&A (2)References (3)

Key features and details

  • Donkey Anti-Mouse IgG H&L (Texas Red ®) preadsorbed
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Donkey
  • Suitable for: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IF

概述

  • 产品名称

    驴抗小鼠IgG H&L (Texas Red ®)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISA, ICC/IFmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native mouse IgG (purified).

  • 偶联物

    Texas Red ®. Ex: 596nm, Em: 620nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • 纯度

    Affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • 共轭说明

    Texas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG

  • 克隆

    多克隆

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7059于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
ICC/IF
1/1000 - 1/5000. PubMed: 18263878
说明
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ELISA
1/10000 - 1/50000.
ICC/IF
1/1000 - 1/5000. PubMed: 18263878

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab7059？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7059 被引用在 3 文献中.

  • Muller-Delp JM  et al. Effects of age and exercise training on coronary microvascular smooth muscle phenotype and function. J Appl Physiol (1985) 124:140-149 (2018). PubMed: 29025901
  • Planutis K  et al. A novel signaling pathway regulates colon cancer angiogenesis through Norrin. Sci Rep 4:5630 (2014). IHC-P ; Human . PubMed: 25005225
  • Hejna J  et al. Tip60 Is Required for DNA Interstrand Cross-link Repair in the Fanconi Anemia Pathway. J Biol Chem 283:9844-51 (2008). ICC/IF . PubMed: 18263878

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

human tissue
intestinal tumor as pos. crtl
no signal at all
4 um slides
AR: citrate buffer (heating plate)
block: 10% donkey serum 30min
ab16832: 5, 10 ug/ml
ab118519: 20 ug/ml

suggested: try other AR, use more Ab, try HRP-secondary + hematoxyline (to check if any signal can be obtained)

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Answer

Thank you for contacting us.
I am sorry to hear that you received so far no staining with the antibodies.

As we discussed by phone, I'd suggest trying the following:

1) try another antigen retrieval step (e.g. Tris/EDTA, pH 8)
2)use more priamry antibody
3) try using an HRP-conjugated secondary antibody and hematoxyline (for nuclear staining)- to check if any signal can be obtained at all, with the experimentalsetup you have.

Right now it is difficult to determine where the problem might stem from - there could be multiple causes. Thus, please let me know what results you obtained after trying these tips and we can discuss this further.

I wish you good luck and look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
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Question

I have a mouse monoclonal which is IgG1kappa. I would like to make sure that the second antibody ab 7059 will recognise my monoclonal. could you please let me know?

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Answer

This antibody will detect IgG1 kappa.

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