Application
Immunocytochemistry/ Immunofluorescence
Mouse brain was perfusion-fixed using 3.7% PFA and paraffin embedded
Primary antibody: Mouse anti-PCNA ab29
Secondary antibody: Donkey anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Abcam ab150109)
Blocking buffer: 5% Donkey serum
Brain section was blocked for 1h at room temperature.
Incubation in primary antibody diluted in blocking buffer (1:10.000 dilution) at 4 °C for 16-18 h.
Incubation in secondary antibody diluted in blocking buffer (1:250 dilution) at room temperature for 2 h.
Primary antibody: Mouse anti-PCNA ab29
Secondary antibody: Donkey anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (Abcam ab150109)
Blocking buffer: 5% Donkey serum
Brain section was blocked for 1h at room temperature.
Incubation in primary antibody diluted in blocking buffer (1:10.000 dilution) at 4 °C for 16-18 h.
Incubation in secondary antibody diluted in blocking buffer (1:250 dilution) at room temperature for 2 h.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Hannes Kaddatz
Verified customer
提交于 Jan 23 2020