驴抗山羊IgG H&L (HRP) (ab97110)

Reviews (4)Q&A (2)References (49)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)

Key features and details

  • Donkey Anti-Goat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, WB, ICC

概述

  • 产品名称

    驴抗山羊IgG H&L (HRP)
    参阅全部 IgG 二抗

  • 宿主

    Donkey

  • 靶标种属

    Goat

  • 特异性

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

  • 经测试应用

    适用于: IHC-P, ELISA, WB, ICCmore details

  • 免疫原

    Other Immunogen Type corresponding to Goat IgG. Contains both the heavy and light chain

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.8
    Constituent: 0.05% CMIT/MIT based preservative
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

  • 共轭说明

    Molar enzyme/ antibody protein ratio is 4:1

  • 克隆

    多克隆

  • 同种型

    IgG

  • 常规说明

    Part of the AbExcel range.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97110于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/200 - 1/500.
ELISA (1)
1/10000 - 1/100000. (Primary).
WB (3)
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC
1/200 - 1/500.
说明
IHC-P
1/200 - 1/500.
ELISA
1/10000 - 1/100000. (Primary).
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC
1/200 - 1/500.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/200 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
    All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 & 5-6 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
    Lanes 3-4 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 minutes

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (49)

发表研究结果有使用 ab97110？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab97110 被引用在 49 文献中.

  • Kumar V  et al. Dual Specific Phosphatase 14 Deletion Rescues Retinal Ganglion Cells and Optic Nerve Axons after Experimental Anterior Ischemic Optic Neuropathy. Curr Eye Res 46:710-718 (2021). PubMed: 33107352
  • Liu H  et al. Erb-B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53-responsive microRNA-3184-5p in cervical cancer cells. Oncol Rep 45:95-106 (2021). PubMed: 33416166
  • Azad T  et al. Implications for SARS-CoV-2 Vaccine Design: Fusion of Spike Glycoprotein Transmembrane Domain to Receptor-Binding Domain Induces Trimerization. Membranes (Basel) 10:N/A (2020). PubMed: 32872641
  • Chang L & Zhu L Dewormer drug fenbendazole has antiviral effects on BoHV-1 productive infection in cell cultures. J Vet Sci 21:e72 (2020). PubMed: 33016019
  • Godavarthy PS  et al. The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis. Haematologica 105:136-147 (2020). PubMed: 31018977
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Direct ELISA method development

 Excellent
Abreviews
abreview image
Application
ELISA
Capture : Purified recombinant protein
1st Detection : Anti-target Goat Ab (1:5000)
2nd Detection : Donkey Anti-goat IgG H&L (1:6000)

Abcam user community

Verified customer

提交于 Sep 17 2020

Excellent 2nd antibody for WB

 Excellent
Abreviews
abreview image
Application
Western blot
A excellent 2nd Ab for WB (1:2,000 dilution) used with ab166930 (1:2000).

Mr. Baptiste Jude

Verified customer

提交于 Jul 06 2016

excellent antibody for WB assay

 Excellent
Abreviews
Application
Western blot
This antibody is good for western blot assay, a dilution of 1 to 5,000 could always offer a good detection signal, which was comparable to the dilution of 1 to 2,000.

Abcam user community

Verified customer

提交于 Nov 05 2015

good 2nd Ab for WB

 Excellent
Abreviews
Application
Western blot
a good 2nd Ab for WB (1:5,000 dilution).

Abcam user community

Verified customer

提交于 Nov 02 2015

Question

I have been using ab97110 as a secondary for my Westerns. However, since we only need such a amount and very dilute, we end up wasting a lot of it. I only am running Westerns semi-regularly and the workload comes in waves, so it possible to aliquot and freeze the antibody for long-term storage? If so, at -20 or -80? Thank you. Sincerely, \

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Answer

We have studied the effect of freeze thaw cycles on conjugated antibodies and have seen that they can have significant effect on HRP secondaries. As such we do not recommend stroage at -20C. These products should have good activity for one year or more when stored at +4C and are covered by our 12 month Abpromise guarantee.

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Question

Sir/ Madam, Sorry to disturb you. Our customer makes a request about ab97110:Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (lot:gr13665-10). He has ordered this antibody recently, and he would like to get the COC. Would you please help him provide the COC? Thank you very much :) Best regards.

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Answer

Thank you for your enquiry. Please find enclosed as requested the CoC for ab97110, lot number GR13665-10. I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

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