驴抗山羊IgG H&L (HRP) (ab97110)
Key features and details
- Donkey Anti-Goat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ELISA, WB, ICC
概述
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产品名称
驴抗山羊IgG H&L (HRP)
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
经测试应用
适用于: IHC-P, ELISA, WB, ICCmore details -
免疫原
Other Immunogen Type corresponding to Goat IgG. Contains both the heavy and light chain
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偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Constituent: 0.05% CMIT/MIT based preservative -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
共轭说明
Molar enzyme/ antibody protein ratio is 4:1 -
克隆
多克隆 -
同种型
IgG -
常规说明
Part of the AbExcel range. -
研究领域
相关产品
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Substrate reagent
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- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
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- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97110于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500.
|
|
| ELISA | (1) |
1/10000 - 1/100000. (Primary).
|
| WB | (3) |
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
|
| ICC |
1/200 - 1/500.
|
| 说明 |
|---|
|
IHC-P
1/200 - 1/500. |
|
ELISA
1/10000 - 1/100000. (Primary). |
|
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated. |
|
ICC
1/200 - 1/500. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/200 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 & 5-6 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
Lanes 3-4 : Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 minutes
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (58)
ab97110 被引用在 58 文献中.
- Kumar V et al. Dual Specific Phosphatase 14 Deletion Rescues Retinal Ganglion Cells and Optic Nerve Axons after Experimental Anterior Ischemic Optic Neuropathy. Curr Eye Res 46:710-718 (2021). PubMed: 33107352
- Liu H et al. Erb-B2 Receptor Tyrosine Kinase 2 is negatively regulated by the p53-responsive microRNA-3184-5p in cervical cancer cells. Oncol Rep 45:95-106 (2021). PubMed: 33416166
- Sui Q et al. Dickkopf 1 impairs the tumor response to PD-1 blockade by inactivating CD8+ T cells in deficient mismatch repair colorectal cancer. J Immunother Cancer 9:N/A (2021). PubMed: 33782107
- Burchill MA et al. Oxidized Low-Density Lipoprotein Drives Dysfunction of the Liver Lymphatic System. Cell Mol Gastroenterol Hepatol 11:573-595 (2021). PubMed: 32961356
- Shi X et al. Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro. J Cell Mol Med 25:2563-2571 (2021). PubMed: 33523599