为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Immunology Immunoglobulins Heavy Chain IgG

驴抗山羊IgG H&L (HRP) (ab6885)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (65)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)

Key features and details

  • Donkey Anti-Goat IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

你可能会感兴趣

IHC
DoubleStain Co-Localization IHC Kit: M&M on rodent tissue (Green/HRP & AP/Red) (ab183275)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
色原
Product image
DAB Substrate Kit (ab64238)

查看更多相关产品

概述

  • 产品名称

    驴抗山羊IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Goat
  • 经测试应用

    适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details
  • 免疫原

    Full length native Goat IgG (purified).

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Horseradish Peroxidase (HRP)
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6885于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Dot blot
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/2500.
说明
Dot blot
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/2500.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)Banerjee et al PLoS One. 2013 Nov 15;8(11):e80940. doi: 10.1371/journal.pone.0080940. eCollection 2013. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistologic images of expression of endometrial PECAM-1/CD31 in (Panel A) controls and (Panel B) IRSM (Idiopathic recurrent spontaneous miscarriage).

    3-5 µm thick sections obtained from formaldehyde fixed, paraffin-embedded human tissue were dehydrated in graded ethanol. After antigen retrieval, slides were incubated for an hour in 3% blocking serum (BSA) in PBS for controlling non-specific binding of primary antibody. The slides were then incubated with goat anti-PECAM 1. Excess primary antibody was washed with PBS and the sections were again incubated with ab6885.

    Labeled cells were visualized with diaminobenzidine (DAB) and sections counterstained with hematoxylin. Next, the slides were dehydrated using series of alcohol gradient and mounted using distrene, tricresyl phosphate (DPX) and xylene.

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)Mavroconstanti et al PLoS One. 2013 Aug 1;8(8):e71445. doi: 10.1371/journal.pone.0071445. Print 2013. Fig S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Expression levels of wild type and variant GFP-CDH13 fusion proteins in HEK-293 cells.

    Panel A: GFP-CDH13 fusion proteins (26 kDa+105 kDa?=?131 kDa) were detected in HEK-293 cells by an antibody against GFP. Mock cells transfected with the empty GFP vector expressed only GFP (26 kDa).

    ab6885 used at a 1:5000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab6885)

    IHC image of beta Actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229,10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6885, 1/250 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)
    Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)

    ab6885 was used at dilution 1/5000 with the primary antibody ab36146 in WB. See the review on ab36146.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (65)

发表研究结果有使用 ab6885?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6885 被引用在 65 文献中.

  • Dong Z  et al. Identification lncRNA LOC102551149/miR-23a-5p pathway in hepatic fibrosis. Eur J Clin Invest 50:e13243 (2020). PubMed: 32306379
  • Wang Y  et al. ELMO2 association with Gai2 regulates pancreatic cancer cell chemotaxis and metastasis. PeerJ 8:e8910 (2020). PubMed: 32292657
  • Hu L  et al. The Influence of Bcl-3 Expression on Cell Migration and Chemosensitivity of Gastric Cancer Cells via Regulating Hypoxia-Induced Protective Autophagy. J Gastric Cancer 20:95-105 (2020). PubMed: 32269848
  • Chen Z  et al. R-spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells. Am J Physiol Cell Physiol 318:C664-C674 (2020). PubMed: 31851527
  • Liu X  et al. Overexpression of the human cytomegalovirus UL111A is correlated with favorable survival of patients with gastric cancer and changes T-cell infiltration and suppresses carcinogenesis. J Cancer Res Clin Oncol 146:555-568 (2020). PubMed: 32025866
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-6 of 6 Abreviews or Q&A

Question

Dear Sir/ Madam,
I am looking for a peroxidase secondary antibody to reacts with goat. The antibody that I am using is ab 13627. In your website I found ab 6741, ab 6885 and ab 97100. Could you please indicate which one is better to react with ab13627? Do you have any other suggestion?
Best regards,

Read More

Abcam community

Verified customer

Asked on Nov 26 2012

Answer

Thank you for contacting us.

As I presume these antibodies will be used in ICC/IF (which is the application ab13627 has been tested in), I would then suggest using ab6885 or ab6741 as secondary antibody.

Both should recognise and be suitable for detecting ab13627. If you had any problem using any of these, please note that all of our products are covered by the Abpromise guarantee, and in case they do not perform as stated on the datasheet we will replace or refund the product.

I’d like to bring your attention to an offer we are running throughout November. If you order any primary antibody you can receive a RabMab free, whilst stocks last. Simply quote “RABMAB-XBSMG” in your next primary antibody order. More information on this offer can be found from the following link:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Please do not hesitate to contact us for further assistance.

Read More

Abcam Scientific Support

回复于 Nov 26 2012

Question

I convinced them to accept the replacements. I believe they will not have problems with the new secondaries, but please make sure the replacements do not have brown precipitates in the vials.

I am looking forward to hearing from you.

Best Regards,

Read More

Abcam community

Verified customer

Asked on Feb 02 2012

Answer

I have requested that you be sent 3 new antibodies for the customer and I have also asked that the vials be checked for brown precipitate before being shipped.

Thank you for all your help with this matter and I hope it all works out.

Please let me know if there is anything else I can help you with.

Read More

Abcam Scientific Support

回复于 Feb 02 2012

Question

I have some bad news. Please, read below what customer answered me about this case:

I would like to inform you that the antibodies were tested in WB experiments and the incubation time and concentrations used are the same that work for other secondary antibodies from other brands and even from Abcam.

The test you suggested, to put 1ul secondary + 500 ul ECL, does not mean the secondary is recognizing the primary, but that the HRP is working.

So, I am sorry to inform you that we do not want to buy more from Sellex and I will then consider to purchase antibodies from other companies (Sigma, Ge, Millipore), as Abcam is not facilitating the exchange of so few antibodies and the laboratory can not lose these reagents.
I hope you send this information to Abcam, and replace the secondaries as fast as possible; we have already informed and testedeverything we could."


I am looking forward to hearing from you.

Read More

Abcam community

Verified customer

Asked on Jan 31 2012

Answer

I am really sorry that the customer is so upset, that was never my intention. I understand that they are well versed in the WB field; howeverI was only trying to get some more information on the protocol that they had used.

If the customers are feeling this strongly about the issue, I am happy to fully refund the cost of their purchase, or if there are prepared to give us another chance, then I can send replacements.

Please let me know whether you would like to me to send replacements, if so, which ones, or if the customer would like a refund.

Read More

Abcam Scientific Support

回复于 Jan 31 2012

Question

\Our customer is still complaining that all these 3 secondaries are not working. The group has experience inWBapplication as this is the major techniqueperformed in their lab.Unfortunatelly, they are very disapointed with these secondaries they received.

Read More

Abcam community

Verified customer

Asked on Jan 24 2012

Answer

Thank you for contacting Abcam.

I am sorry that your customer is still having problems with these antibodies. As I mentioned in my previous email in November, could you provide me with the protocol that they are using and I can see if there is any information that I can give that will help.

Do the primary antibodies that they are using work when they use them with different secondary antibodies (not the ones they are having issues with?).

The antibodiesare covered under our Abpromise for six months and is guaranteed to work in western blot . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. However, we do need to have some description of the problem before we can issue you any replacement or a refund.

I look forward to your response and resolving this issue.

Read More

Abcam Scientific Support

回复于 Jan 24 2012

Question

For the technical support,   Customer has reported brown precipitates in the vials below, and after testing none of them worked. I suggested vortexing the vials as recommended on the datasheet, but nothing changed, customer says the antibodies are not working.    ab6721 (2 units) lot GR27131-2 ab6789 (2 units) lot GR23382-2 ab6885 (1 unit) lot GR34440-1 "The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal."   I am providing the order number and I look forward to hearing from you.  

Read More

Abcam community

Verified customer

Asked on Nov 15 2011

Answer

I am sorry that you are experiencing problems with the HRP tagged antibodies that you purchased from us. As you mentioned in the email that was sent, it is common for hrp tagged secondaries to have a brown precipitate in the vial, but vortexing should allow the antibody to work as stated. I was wondering if you could give me some more information on how you tested the antibodies, application, incubation times, concentration of antibody used. Also, there is a way to test the reactivity of hrp tagged secondaries. Take 500ul of your mixed ECL reagent into the dark room and then add 1ul of your secondary antibody into the tube. If the secondary antibody is active then the solution will turn a fluorescent blue. Have you tried this with the antibodies? Unfortunately the order number that was used to purchase these products did not come I look forward to you reply and helping you resolve this issue.

Read More

Abcam Scientific Support

回复于 Nov 15 2011

Question

Hej ! What's the optimal dilution for this ab ? (8. Dilute the horseradish peroxidase labeled secondary antibody at the optimal dilution (according to the manufacturer) in blocking buffer.)

Read More

Abcam community

Verified customer

Asked on Jun 27 2001

Answer

We recommend the follwing dilutions: ELISA 1:4,000 - 1:10,000 Immunoblotting 1:2,000 - 1:5,000 Immunohistochemistry 1:200 - 1:2,000

Read More

Abcam Scientific Support

回复于 Jun 28 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2021 艾博抗(上海)贸易有限公司版权所有