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Immunology Immunoglobulins Heavy Chain IgG

驴抗山羊IgG H&L (Cy5 ®)预吸附二抗(ab6566)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (34)

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Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
  • Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)

Key features and details

  • Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
  • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISA

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy3 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated

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概述

  • 产品名称

    驴抗山羊IgG H&L (Cy5 ®)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Goat
  • 经测试应用

    适用于: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
  • 最小交叉反应


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Goat IgG, whole molecule

  • 偶联物

    Cy5 ®. Ex: 650nm, Em: 667nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • 共轭说明

    Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Cy5

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6566于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.
说明
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent dilution.
ICC/IF
1/1000 - 1/5000.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent dilution.
ELISA
1/10000 - 1/50000.

图片

  • Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
    Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).

    Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.

    Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).

  • Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
    Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)This image is courtesy of an anonymous Abreview
    Lanes 2-3 : Goat anti-UCP1 at 1/200 dilution

    Lane 1 : Marker
    Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg

    Secondary
    Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution

    Observed band size: 33 kDa why is the actual band size different from the predicted?

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (34)

发表研究结果有使用 ab6566?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6566 被引用在 34 文献中.

  • Zhao LM  et al. Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells. Bioact Mater 14:206-218 (2022). PubMed: 35310356
  • Lennikov A  et al. Deficiency of C-X-C chemokine receptor type 5 (CXCR5) gene causes dysfunction of retinal pigment epithelium cells. Lab Invest 101:228-244 (2021). PubMed: 32994482
  • Liu X  et al. The protective role of miR-132 targeting HMGA2 through the PI3K/AKT pathway in mice with Alzheimer's disease. Am J Transl Res 13:4632-4643 (2021). PubMed: 34150043
  • Zhang S  et al. Inhibition of MALT1 Alleviates Spinal Ischemia/Reperfusion Injury-Induced Neuroinflammation by Modulating Glial Endoplasmic Reticulum Stress in Rats. J Inflamm Res 14:4329-4345 (2021). PubMed: 34511971
  • Sun C  et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

abreview for ab6566 - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
All lanes: Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) 1:2500
Lane 1: Marker
Lane 2: Protein lysate of BAT (Mouse 1), Protein 70µg
Lane 3: Protein lysate of BAT (Mouse 2), Protein 70µg
Lanes 2+3: Primary Antibody anti-UCP1 1:200
Predicted band size 33kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 20 2014

Question

The product desription describes this as being Cy5 antibody. The conjugation notes describe this as being Cy5.2 conjugated. Could you please confirm the label and structure of the fluorophore?

Read More

Abcam community

Verified customer

Asked on Aug 03 2007

Answer

Thank you for your enquiry. I believe Cy5.29 OSu is the same as Cy5, but refers more specifically to the cyanine derivative, a succinimide ester, that is used for conjugation. The following paper describes this ester: Cytometry. 1990;11(3):418-30. PMID: 2340776 I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Aug 03 2007

Question

I would like to use this donkey anti-goat secondary for a dual staining flow cytomytry experiment. The other secondary is goat anti-mouse IgG so I was wondering if the donkey anti-goat would recognize the other secondary or would the fluorphore's linked to the goat anti-mouse secondary mask any potential epitopes. Thanks

Read More

Abcam community

Verified customer

Asked on Jun 15 2002

Answer

This antibody will indeed recognise the other secondary. You need to use a secondary antibody to the mouse IgG raised in a species other than goat, preferably donkey (if you want to use ab6566). Look for a donkey anti-mouse IgG fluorphore conjugated secondary antibody on the Abcam website.

Read More

Abcam Scientific Support

回复于 Jun 17 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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