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Immunology Immunoglobulins Heavy Chain IgG

驴抗山羊IgG H&L (Cy3 ®)预吸附二抗(ab6949)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (28)

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Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)

    Key features and details

    • Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
    • Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
    • Host species: Donkey
    • Isotype: IgG
    • Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WB

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alexa Fluor® 790 Alkaline Phosphatase Biotin Cy2 ® Cy5 ® Cy5.5 ® DyLight® 488 DyLight® 550 DyLight® 594 DyLight® 650 FITC Gold 10nm Gold 12nm Gold 18nm Gold 25nm HRP HRP polymer IRDye® 800CW PE Texas Red ® TRITC Unconjugated Unconjugated Unconjugated

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    概述

    • 产品名称

      驴抗山羊IgG H&L (Cy3 ®)预吸附二抗
      参阅全部 IgG 二抗
    • 宿主

      Donkey
    • 靶标种属

      Goat
    • 经测试应用

      适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details
    • 最小交叉反应


      Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat
      To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
      more details
    • 免疫原

      Full length native Goat IgG (purified).

    • 偶联物

      Cy3 ®. Ex: 552nm, Em: 565nm

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      pH: 6.50
      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
    • 共轭说明

      Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG
    • 克隆

      多克隆
    • 同种型

      IgG
    • 常规说明

      This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


      This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

    • 研究领域

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Secondary antibodies
      • anti-Goat
      • IgG
      • Fluorophore
      • Cy3

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6949于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr (1)
    Use at an assay dependent concentration.
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    WB
    1/1000. Predicted molecular weight: 36 kDa.
    说明
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    WB
    1/1000. Predicted molecular weight: 36 kDa.

    图片

    • Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
      Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
      Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 cells + Goat IgG, 50 ng

      Predicted band size: 36 kDa



      Both the heavy and light chains of Goat IgG are observed.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (28)

    发表研究结果有使用 ab6949?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6949 被引用在 28 文献中.

    • Tian C & Johnson KR TBX1 is required for normal stria vascularis and semicircular canal development. Dev Biol 457:91-103 (2020). PubMed: 31550482
    • Gao L  et al. Vps35 Deficiency Impairs Cdk5/p35 Degradation and Promotes the Hyperphosphorylation of Tau Protein in Retinal Ganglion Cells. Invest Ophthalmol Vis Sci 61:1 (2020). PubMed: 31995153
    • Mahameed M  et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
    • Cooke JE  et al. Contrast gain control occurs independently of both parvalbumin-positive interneuron activity and shunting inhibition in auditory cortex. J Neurophysiol 123:1536-1551 (2020). PubMed: 32186432
    • Srivastava Y  et al. Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17. FEBS J 287:122-144 (2020). PubMed: 31569299
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Excellent secondary antibody for fresh frozen tissue sections

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Tissue: Acute wound

    Fix & permeabilization: Acetone

    Blocking: 5% BSA/PBS

    1st antibody: anti-CD81 goat antibody (1/20 PBS)

    2nd antibody: Donkey Anti-Goat IgG H&L (Cy3) preadsorbed (ab6949)(1/1000 PBS)

    The anti goat antibody gives very specific and strong signals on frozen-tissue sections

    Abcam user community

    Verified customer

    提交于 Jul 22 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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