驴抗山羊IgG H&L (Cy3 ®)预吸附二抗(ab6949)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed
- Conjugation: Cy3 ®. Ex: 552nm, Em: 565nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WB
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HRP
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IRDye® 800CW
PE
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TRITC
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概述
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产品名称
驴抗山羊IgG H&L (Cy3 ®)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
经测试应用
适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details -
最小交叉反应
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
免疫原
Full length native Goat IgG (purified).
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偶联物
Cy3 ®. Ex: 552nm, Em: 565nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
共轭说明
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG -
克隆
多克隆 -
同种型
IgG -
常规说明
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/1000. Predicted molecular weight: 36 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/1000. Predicted molecular weight: 36 kDa. |
图片
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Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 cells + Goat IgG, 50 ng
Predicted band size: 36 kDaBoth the heavy and light chains of Goat IgG are observed.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab6949 被引用在 28 文献中.
- Tian C & Johnson KR TBX1 is required for normal stria vascularis and semicircular canal development. Dev Biol 457:91-103 (2020). PubMed: 31550482
- Gao L et al. Vps35 Deficiency Impairs Cdk5/p35 Degradation and Promotes the Hyperphosphorylation of Tau Protein in Retinal Ganglion Cells. Invest Ophthalmol Vis Sci 61:1 (2020). PubMed: 31995153
- Mahameed M et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun 11:1304 (2020). PubMed: 32161259
- Cooke JE et al. Contrast gain control occurs independently of both parvalbumin-positive interneuron activity and shunting inhibition in auditory cortex. J Neurophysiol 123:1536-1551 (2020). PubMed: 32186432
- Srivastava Y et al. Cancer-associated missense mutations enhance the pluripotency reprogramming activity of OCT4 and SOX17. FEBS J 287:122-144 (2020). PubMed: 31569299