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Immunology Immunoglobulins Heavy Chain IgG

驴抗山羊IgG H&L (Alexa Fluor® 488) (ab150129)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (116)

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Flow Cytometry - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

Key features and details

  • Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

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Product image
Normal Donkey Serum (ab7475)

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概述

  • 产品名称

    驴抗山羊IgG H&L (Alexa Fluor® 488)
    参阅全部 IgG 二抗
  • 描述

    驴多克隆抗体二抗to山羊IgG - H&L (Alexa Fluor® 488)
  • 宿主

    Donkey
  • 靶标种属

    Goat
  • 特异性

    This antibody is specific to Goat IgG
  • 经测试应用

    适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • 免疫原

    Full length protein corresponding to Goat IgG.

  • 偶联物

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Goat
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

相关产品

  • Related Products

    • Normal Donkey Serum (ab7475)

应用

Our Abpromise guarantee covers the use of ab150129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000 - 1/4000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

图片

  • Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

    ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H+L) (shown in green) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Flow Cytometry - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    Flow Cytometry - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

    Overlay histogram showing Jurkat cells stained Goat polyclonal to Ikaros (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Goat polyclonal to Ikaros, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 donkey anti-goat IgG (H&L) (ab150129) at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was goat IgG (polyclonal) (ab37373, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (116)

    发表研究结果有使用 ab150129?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab150129 被引用在 116 文献中.

    • Wang Z  et al. Neurogenic Niche Conversion Strategy Induces Migration and Functional Neuronal Differentiation of Neural Precursor Cells Following Brain Injury. Stem Cells Dev 29:235-248 (2020). PubMed: 31797735
    • Wang H  et al. Natural Heart Regeneration in a Neonatal Rat Myocardial Infarction Model. Cells 9:N/A (2020). PubMed: 31963369
    • Kovács D  et al. Core-shell nanoparticles suppress metastasis and modify the tumour-supportive activity of cancer-associated fibroblasts. J Nanobiotechnology 18:18 (2020). PubMed: 31964403
    • Lauenstein JU  et al. Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain. J Immunol 204:2269-2276 (2020). PubMed: 32198144
    • Morse SV  et al. Neuron labeling with rhodamine-conjugated Gd-based MRI contrast agents delivered to the brain via focused ultrasound. Theranostics 10:2659-2674 (2020). PubMed: 32194827
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Excellent secondary antibody for FFPE

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    This secondary antibody was used to stain vimentin in human skin sections (nevus).
    Concentration: 1:500
    Very clear and specific staining

    Abcam user community

    Verified customer

    提交于 Jun 17 2020

    Vimentin staining of melanoma cells by indirect IF

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Cell: Sk-Mel28

    Fix & permeabilization: Acetone

    Blocking: 5% BSA/PBS

    1st antibody: anti-Vimentin goat polyclonal antibody (1/100 PBS)

    2nd antibody: Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) (1/500 PBS)

    Abcam user community

    Verified customer

    提交于 Jun 01 2020

    TIMP1 expression in human skin

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    This antibody was used to study the TIMP1-expression in human skin tissue (melanoma vs nevus) by indirect immunfluorescence.
    The anti goat antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results.
    Concentration 1:200

    Abcam user community

    Verified customer

    提交于 Mar 04 2019

    Labeling of granule cells in the Dentate Gyrus of the Mouse Hipocampus

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA fixed)
    Very good antibody

    Yacine Tensaouti

    Verified customer

    提交于 Aug 01 2017

    Question

    I want to use your ab150114 and ab150129 to do co-localization test. But I notice that the emission wavelength of these two antibodies overlap quite a lot. Will that affect my result? At the beginning I want to buy Alexa 488 and Alexa 594 to do the experiment, but the problem is our lab don't have the lsm which can detect Alexa 594 (the machine only has laser 405, 488 and 543, and I find the ex of Alexa 594 is 615nm). Could you give me any suggestions? I want to know whether it will work if I am going to buy Alex 488 and 555.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2013

    Answer

    For excitation with the 543nm laser, Alexa Fluor 568 is a better choice than 555 for combination with AF488. There is still some emission spillover but it is much less than for the 488/555 combination, and you should be able to set compensation without as much trouble. If the 488 signal is expected to be much brighter than the other, for instance if that protein is much more abundant than the other, then spillover may still be an issue. For an anti-mouse IgG secondary, I suggest ab175473.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    回复于 Oct 24 2013

    Brachyury expression in spontaneously differentiated human iPS cells.

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Following fixation in 4% PFA, the cells were assessed for Brachyury (T) expression using 1:100 dilution of the primary antibody in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using donkey polyclonal rabbit IgG Alexa 488 (ab150129) at 1:500. The results show that nuclear T (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here). All nuclei were counterstained using DAPI (blue).

    Abcam user community

    Verified customer

    提交于 Sep 02 2013

    Question

    I am getting significant signal with this antibody diluted 1:1000 in mouse 3T3L1 (preadipocytes) without the addition of any primary antibody. I fix these cells in 4% PFA for 15 minutes and wash 3Xs 10 minutes. Further, I have blocked using 5% donkey serum with 1% BSA, 10% donkey serum, and 10% horse serum for 1 hour at room temperature all with similar results. Any other suggestions?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 04 2013

    Answer

    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab150129. I would also appreciate if you can confirm some further details: Do you use a detergent such as Tween 20 in your washing buffer? Have you attempted to increase the number and duration of washes after antibody incubation? If so, did this have affect on the background? Have you attempted to further dilute the antibody to 1/1500, 1/2000 or greater? This antibody has not been pre-absorbed against mouse IgG, as such we cannot guarantee that we will be able to eliminate this reactive thought we hope that these suggestions will help reduce this. Abcam does offer a number of AlexaFluor 488 antibodies which have been pre-absorbed to ensure low species cross-reactivity including ab150133 which has been pre-absorbed against chicken, human, mouse, pig, rabbit and rat. More details about this product may be found at: https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-hl-alexa-fluorreg-488-pre-adsorbed-ab150133.html

    Read More

    Abcam Scientific Support

    回复于 Apr 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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