驴抗山羊IgG H&L (6nm Gold)预吸附二抗(ab105268)

概述

  • 产品名称
    驴抗山羊IgG H&L (6nm Gold)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主
    Donkey
  • 靶标种属
    Goat
  • 经测试应用
    适用于: ELISA, Electron Microscopy, WBmore details
  • 最小交叉反应

    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details
  • 偶联物
    Gold 6nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 8.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.381% Sodium borate, 1.5% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab105268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

文献

This product has been referenced in:
  • Ferrer-Acosta Y  et al. EFhd2 is a novel amyloid protein associated with pathological tau in Alzheimer's disease. J Neurochem 125:921-31 (2013). Read more (PubMed: 23331044) »
See 1 Publication for this product

客户评价及客户问答

1-2 of 2 Q&A

Answer

Thank you for your reply.
I am sorry for the confusion about the concentration for this antibody and the recommended starting concentrations to use.
Even though the concentration of 0.1ug/ml is no longer valid in this case, we still guarantee the antibody to work in EM. As with all of our recommended dilutions on the datasheet, these are meant as a guide for the customer to know what a good starting point is, but the optimal dilutions/concentrations should be determined by the end user.
As you mentioned, I did make a mistake in the dilution I sent you and I do apologize for that and you should definitely use it at a higher dilution. Any publications that we know of that have used this antibody would be posted on the website. Currently, we do not have any.
If you would like to return the antibody there will be a 20% restocking fee that will be applied and we do ask that it is shipped overnight on an ice pack, with all the original documentation and that we receive it no more than 7 days after it arrived.
Please let me know if you would like to return the product and I will process the refund and send you the appropriate details.

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Answer

Thank you for contacting Abcam.
Unfortunately we do not know the antibody concentration in gold complexes because we separate monomers from multimers on density gradients. We do not have any reliable way to measure the antibody concentration on the monomers because the preparation is stabilized with albumin before and after centrifugation.
I believe that the recommended starting concentration of 0.1ug/ml was added in error and I am having our datasheet team look into this and make any necessary changes. For your experiments, I would recommend that you try a 1/5000 dilution to begin with. You may have to alter this for future experiments depending on what your initial results show, but it would be a good starting point.
Please let me know if there is anything else I can help you with.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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