为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Immunology Immunoglobulins Heavy Chain IgG

驴F(ab')2抗羊IgG H&L (PE)预吸附二抗(ab7009)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (1)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Donkey F(ab')2 Anti-Sheep IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt

你可能会感兴趣

偶联
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
封闭
Product image
Normal Donkey Serum (ab7475)

查看更多相关产品

概述

  • 产品名称

    驴F(ab')2抗羊IgG H&L (PE)预吸附二抗
    参阅全部 IgG 二抗
  • 宿主

    Donkey
  • 靶标种属

    Sheep
  • 经测试应用

    适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应


    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Sheep IgG whole molecule

  • 偶联物

    PE. Ex: 488nm, Em: 575nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Prepared from monospecific antiserum by immunoaffinity chromatography using sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 共轭说明

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Sheep
    • IgG
    • Fluorophore
    • Phycoerythrin

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7009于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
说明
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab7009?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7009 被引用在 1 文献中.

  • Tanaka Y  et al. Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation. Stem Cells Dev 17:367-81 (2008). Flow Cyt . PubMed: 18447651

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Question

Antibody seems not to perform recognizing serum opsonised beads or lateral flow capture lines that get a strong signal when using monoclonal anti sheep FITC antibody. Antibodies in lateral flow were also not recognised by anti-lambda ovine antibody so potentially the polyclonal recognises preferentially lambda which would be correct as sheep have 99% lambda antibodies. It would be worthwhile testing this out and put a warning onto the use of the antibody.

Read More

Abcam community

Verified customer

Asked on Mar 23 2012

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody in lateral flow experiments.

We appreciate your valuable feedback. Regrettably, this antibody has not been tested in lateral flow, so we would unfortunately not be able to guarantee how well it will work in this case.

I would like to investigate this particular case for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you are able to provide some further information on the experiments:

1. Please provide more detail regarding what type of samples were tested?
2. What was the protocol used?
3. I would appreciate if you could also provide any images which would help us to assess the results.

Also, could you confirm if the antibody has been tried in a tested application? I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytometry. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Thank you for your time and cooperation. We look forward to receiving the requested information.

Read More

Abcam Scientific Support

回复于 Mar 23 2012

Question

Thank you for your response, please see below the response from the customer;

I thought I had explained myself clearly. I am well aware that these are donkey derived antibodies! I am using them against sheeppolyclonal antibodies (purified to contain onlyIgG). The sheep polyclonals are just attached to beads. The beads are then measured for PE fluorescence. The fact is that the same product made byanother company seems to work quite well whereas the Abcam one does not.

I'm not sure what other information I can give, but I'm pretty disappointed with that response from Abcam.

Kind regards,

Read More

Abcam community

Verified customer

Asked on May 08 2012

Answer

Thank you for your response and the further clarification.

I am afraid this information was not provided in the initial e-mail.

Your credit note ID is 20806.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Abcam Scientific Support

回复于 May 08 2012

Question

My apologies! Sorry that was pretty silly of me to forget the catalogue number .. K


It is ab7009 Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed

Please see below for an email we received from a customer;

I have tested this product (twice) and was disappointed with the results. When compared to a 4 year old vial of the equivalent Santa Cruz product, the Abcam antibody produced about 1/10th the signal. The application was a validation of sheep polyclonal antibody coupled beads, used for and read by a Bio-plex instrument (basically measuring the amount of sheep antibodies bound to the beads). All conditions in the experiment were the same, including test sample and antibody concentration.


Lot #: GR38595

Sapphire order number: PO-13364
Abcam invoice number : 1315698B



Sorry again!



Hope that helps!



Kind regards,

Read More

Abcam community

Verified customer

Asked on May 04 2012

Answer

My colleague is out of office and she has asked me to look after her customers in her absence.

Thank you for response and for confirming the product code, the batch and the order number> I am a bit confused about this enquiry since ab7009 is a donkey polyclonal antibody and not sheep, so it contains donkey IgG (F(ab')2) - fragment.

It was raised against sheep IgG so the immunogne is sheep IgG whole molecule but the actual product consists of donkey immunoglobulins.

Furthermore, ab7009 has not been tested in IP or any separation technique which requires beads. It has been characterized ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Abcam Scientific Support

回复于 May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2022 艾博抗(上海)贸易有限公司版权所有