驴F(ab')2抗兔IgG H&L (PE)预吸附二抗(ab7007)
Key features and details
- Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cyt
概述
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产品名称
驴F(ab')2抗兔IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG whole molecule
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Prepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins -
共轭说明
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
克隆
多克隆 -
同种型
IgG -
常规说明
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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研究领域
应用
应用 | Ab评论 | 说明 |
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IHC-P |
1/200.
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ICC/IF |
1/100 - 1/250.
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Immunomicroscopy |
Use at an assay dependent dilution.
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Flow Cyt |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
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说明 |
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IHC-P
1/200. |
ICC/IF
1/100 - 1/250. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab7007 被引用在 26 文献中.
- Tian L et al. IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application. Mol Ther Oncolytics 20:187-198 (2021). PubMed: 33665357
- Li J et al. SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer. Oncogene 40:4884-4893 (2021). PubMed: 34163029
- Harati R et al. Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling. Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 32645833
- Harati R et al. Silencing miR-202-3p increases MMP-1 and promotes a brain invasive phenotype in metastatic breast cancer cells. PLoS One 15:e0239292 (2020). PubMed: 33002044
- Santos ACA et al. Prostaglandin F2a in vitro can affect basic inflammatory parameters of mesenchymal stem cells and slight modulating some of their immunomodulatory properties. Prostaglandins Leukot Essent Fatty Acids 163:102210 (2020). PubMed: 33242781