Key features and details
- Donkey F(ab) Anti-Goat IgG H&L (TRITC)
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISA
产品名称驴F(ab)抗山羊IgG H&L (TRITC)
参阅全部 IgG 二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
经测试应用适用于: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
Goat IgG whole molecule
偶联物TRITC. Ex: 547nm, Em: 572nm
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
共轭说明Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.2 moles TRITC per mole of Donkey IgG Fab
Our Abpromise guarantee covers the use of ab6522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.