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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-DOK2抗体(ab1677)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab1677)

    Key features and details

    • Rabbit polyclonal to DOK2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-DOK2抗体
      参阅全部 DOK2 一抗
    • 描述

      兔多克隆抗体to DOK2
    • 宿主

      Rabbit
    • 特异性

      Binds to a 55-60 kDa protein.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • 免疫原

      20 amino acid C-terminal peptide to p56 dok2 RasGAP-binding protein

    • 阳性对照

      • Human U937 cells or T-cell line Jurkatt.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: 0.1% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Cancer
      • Signal transduction
      • Other
      • Cancer
      • Signal transduction
      • Protein phosphorylation
      • Tyrosine kinases
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1677 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    IHC-P
    Human
    All applications
    Mouse
    应用 Ab评论 说明
    IHC-P
    Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    靶标

    • 功能

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
    • 组织特异性

      Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
    • 序列相似性

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 结构域

      PTB domain mediates receptor interaction.
    • 翻译后修饰

      On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
    • Target information above from: UniProt accession O60496 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 9046 Human
      • Entrez Gene: 13449 Mouse
      • Omim: 604997 Human
      • SwissProt: O60496 Human
      • SwissProt: O70469 Mouse
      • Unigene: 71215 Human
      • Unigene: 243323 Mouse
      • 别名

        • Docking protein 2 56kDa antibody
        • Docking protein 2 antibody
        • DOK 2 antibody
        • DOK R antibody
        • DOK2 antibody
        • DOK2_HUMAN antibody
        • Downstream of tyrosine kinase 2 antibody
        • OTTHUMP00000161710 antibody
        • OTTHUMP00000224923 antibody
        • p56(dok 2) antibody
        • p56(dok-2) antibody
        • p56dok 2 antibody
        • p56DOK antibody
        • p56dok2 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab1677)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab1677)
        Ab1677 staining human breast carcinoma. Staining is localized to nucleus and cytoplasm.
        Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
        Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      实验方案

      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献 (0)

      发表研究结果有使用 ab1677?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1677 尚未被引用在任何文献中。

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-DOK2 antibody

      Inconclusive
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (NMuMG)
      Specification
      NMuMG
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% Triton X100
      Blocking step
      BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      提交于 Jun 16 2008

      Western blot abreview for Anti-DOK2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (NMuMG)
      Loading amount
      500000 cells
      Specification
      NMuMG
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      提交于 Jun 03 2008

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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