Anti-DOK2抗体(ab1677)
Key features and details
- Rabbit polyclonal to DOK2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DOK2抗体
参阅全部 DOK2 一抗 -
描述
兔多克隆抗体to DOK2 -
宿主
Rabbit -
特异性
Binds to a 55-60 kDa protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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免疫原
20 amino acid C-terminal peptide to p56 dok2 RasGAP-binding protein
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阳性对照
- Human U937 cells or T-cell line Jurkatt.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| IHC-P |
Human
|
| All applications |
Mouse
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation. -
组织特异性
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver. -
序列相似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
结构域
PTB domain mediates receptor interaction. -
翻译后修饰
On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. - Information by UniProt
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数据库链接
- Entrez Gene: 9046 Human
- Entrez Gene: 13449 Mouse
- Omim: 604997 Human
- SwissProt: O60496 Human
- SwissProt: O70469 Mouse
- Unigene: 71215 Human
- Unigene: 243323 Mouse
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别名
- Docking protein 2 56kDa antibody
- Docking protein 2 antibody
- DOK 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab1677)Ab1677 staining human breast carcinoma. Staining is localized to nucleus and cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
文献 (0)
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