Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Astatotilapia burtoni Tissue sections (thalamus/hypothalamus)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% TritonX
Specification
thalamus/hypothalamus
Blocking step
10% normal serum 1% BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 1% BSA
Dilution
1/100
Additional data
Additional Notes
Staining was much lower than expected. It worked but I have a feeling it would have worked better with an antigen retrieval step.
PubMed ID
N/A: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sebastian Alvarado
Verified customer
提交于 Dec 18 2015