Application
Western blot
Sample
Astatotilapia burtoni Cell lysate - whole cell (Brain and gonads)
Gel Running Conditions
Reduced Denaturing (5% acrylamide gel)
Loading amount
25 µg
Specification
Brain and gonads
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Skim milk
Dilution
1/1000
Detection
Detection method
ECL
Negative control
Gonad extracts (low expression of DNMT3A transcript)
Exposure
15 hour(s), 15 minute(s) and 0 second(s)
Bands
Specific: ~110 kDa
Positive control
Brain extracts (overexpression of DNMT3A transcript)
Additional data
Additional Notes
The cross species reactivity of this antibody is awesome. However, incubation times were particularly long compared to what I would expect had we done this in a Mouse/Human sample
Lane 1: protein ladder
Lane 2: empty
Lane 3: Brain whole cell lysate
Lane 4: Gonad whole cell lysate
PubMed ID
N/A: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Sebastian Alvarado
Verified customer
提交于 Aug 07 2015