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Epigenetics and Nuclear Signaling DNA methylation DNMTs

Anti-Dnmt3a抗体(ab2850)

  • Datasheet
Reviews (11) Submit a question References (76)

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Western blot - Anti-Dnmt3a antibody (ab2850)
  • Western blot - Anti-Dnmt3a antibody (ab2850)

Key features and details

  • Rabbit polyclonal to Dnmt3a
  • Suitable for: WB
  • Reacts with: Mouse, Human, Drosophila melanogaster
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Dnmt3a抗体
    参阅全部 Dnmt3a 一抗
  • 描述

    兔多克隆抗体to Dnmt3a
  • 宿主

    Rabbit
  • 特异性

    This antibody detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1.
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human Dnmt3a aa 86-100.
    Sequence:

    DLLPNGDLEKRSEPQ


    (Peptide available as ab4921)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • DNMTs

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Dnmt3a peptide (ab4921)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Dnmt3a protein (ab170408)

应用

Our Abpromise guarantee covers the use of ab2850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa).Can be blocked with Dnmt3a peptide (ab4921).

靶标

  • 功能

    Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • 组织特异性

    Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • 序列相似性

    Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • 结构域

    The PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻译后修饰

    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • 细胞定位

    Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Target information above from: UniProt accession Q9Y6K1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1788 Human
    • Entrez Gene: 13435 Mouse
    • Omim: 602769 Human
    • SwissProt: Q9Y6K1 Human
    • SwissProt: O88508 Mouse
    • Unigene: 515840 Human
    • Unigene: 5001 Mouse
    • 别名

      • DNA (cytosine 5) methyltransferase 3 alpha antibody
      • DNA (cytosine 5) methyltransferase 3A antibody
      • DNA (cytosine-5)-methyltransferase 3A antibody
      • DNA cytosine methyltransferase 3A2 antibody
      • DNA methyltransferase 3 alpha antibody
      • DNA methyltransferase 3a antibody
      • DNA methyltransferase HsaIIIA antibody
      • DNA MTase HsaIIIA antibody
      • DNM3A_HUMAN antibody
      • DNMT 3a antibody
      • DNMT antibody
      • Dnmt3a antibody
      • DNMT3A2 antibody
      • M.HsaIIIA antibody
      • MCMT antibody
      • OTTHUMP00000201149 antibody
      • TBRS antibody
      see all

    图片

    • Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot - Anti-Dnmt3a antibody (ab2850)Image from Nucleic Acids Res. 2014 Jan; 42(2): 804–821. Fig 5D, doi: 10.1093/nar/gkt920 Reproduced under the creative commons licence. https://creativecommons.org/licenses/by/4.0/

      Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.

    • Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot - Anti-Dnmt3a antibody (ab2850)
      Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (76)

    发表研究结果有使用 ab2850?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2850 被引用在 76 文献中.

    • Peng X  et al. Long non-coding RNA TNRC6C-AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway. J Cell Mol Med 24:304-316 (2020). PubMed: 31657132
    • Zhang S  et al. LncRNA HOTAIR-mediated MTHFR methylation inhibits 5-fluorouracil sensitivity in esophageal cancer cells. J Exp Clin Cancer Res 39:131 (2020). PubMed: 32653028
    • Gao L  et al. Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms. Nat Commun 11:3355 (2020). PubMed: 32620778
    • Jiang Z  et al. Long non-coding RNA LINC00673 silencing inhibits proliferation and drug resistance of prostate cancer cells via decreasing KLF4 promoter methylation. J Cell Mol Med 24:1878-1892 (2020). PubMed: 31881124
    • Jiao J  et al. AID assists DNMT1 to attenuate BCL6 expression through DNA methylation in diffuse large B-cell lymphoma cell lines. Neoplasia 22:142-153 (2020). PubMed: 32062068
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Guinea pig Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Reduced Denaturing (Concentrating 4%, Separating 6%)
    Loading amount
    50 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 25 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton
    Specification
    Spinal cord
    Blocking step
    5% Donkey Serum, 3% BSA as blocking agent for 30 minute(s) · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Menglu Qian

    Verified customer

    提交于 Aug 01 2019

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (HEK and NRK)
    Gel Running Conditions
    Reduced Denaturing (6)
    Loading amount
    20 µg
    Specification
    HEK and NRK
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (dorsal root ganglion and spinal cord (P4))
    Permeabilization
    Yes - 0.2% Triton
    Specification
    dorsal root ganglion and spinal cord (P4)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Franziska Denk

    Verified customer

    提交于 Mar 22 2017

    ChIP abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Breast cancer line)
    Specification
    Breast cancer line
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 14 2017

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cancer line)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    100000 cells
    Specification
    Breast cancer line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 07 2017

    Western blot abreview for Anti-Dnmt3a antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Gonad)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    75 µg
    Specification
    Gonad
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 11 2015

    ChIP abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - other (Epithelial cells)
    Negative control
    IgG
    Specification
    Epithelial cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Positive control
    Input DNA
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 30 2015

    ChIP abreview for Anti-Dnmt3a antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Rat Tissue lysate - whole (Gonad)
    Specification
    Gonad
    Negative control
    IgG
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Positive control
    Input DNA
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 07 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dnmt3a antibody - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample
    Marmoset (common) Tissue sections (Adult testis)
    Specification
    Adult testis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Dr. 煜欽 林

    Verified customer

    提交于 Jan 21 2014

    1-10 of 11 Abreviews or Q&A

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