Anti-Dnmt3a抗体(ab2850)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: WB
- Reacts with: Mouse, Human, Drosophila melanogaster
- Isotype: IgG
概述
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产品名称
Anti-Dnmt3a抗体
参阅全部 Dnmt3a 一抗 -
描述
兔多克隆抗体to Dnmt3a -
宿主
Rabbit -
特异性
This antibody detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human, Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to Human Dnmt3a aa 86-100.
Sequence:DLLPNGDLEKRSEPQ
(Peptide available asab4921) -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa).Can be blocked with Dnmt3a peptide (ab4921). |
靶标
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功能
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
组织特异性
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
序列相似性
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
结构域
The PWWP domain is essential for targeting to pericentric heterochromatin. -
翻译后修饰
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
细胞定位
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 1788 Human
- Entrez Gene: 13435 Mouse
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- SwissProt: O88508 Mouse
- Unigene: 515840 Human
- Unigene: 5001 Mouse
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别名
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
图片
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Western blot - Anti-Dnmt3a antibody (ab2850)Image from Nucleic Acids Res. 2014 Jan; 42(2): 804–821. Fig 5D, doi: 10.1093/nar/gkt920 Reproduced under the creative commons licence. https://creativecommons.org/licenses/by/4.0/
Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.
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Western blot - Anti-Dnmt3a antibody (ab2850)Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.
数据表及文件
文献 (76)
ab2850 被引用在 76 文献中.
- Peng X et al. Long non-coding RNA TNRC6C-AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway. J Cell Mol Med 24:304-316 (2020). PubMed: 31657132
- Zhang S et al. LncRNA HOTAIR-mediated MTHFR methylation inhibits 5-fluorouracil sensitivity in esophageal cancer cells. J Exp Clin Cancer Res 39:131 (2020). PubMed: 32653028
- Gao L et al. Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms. Nat Commun 11:3355 (2020). PubMed: 32620778
- Jiang Z et al. Long non-coding RNA LINC00673 silencing inhibits proliferation and drug resistance of prostate cancer cells via decreasing KLF4 promoter methylation. J Cell Mol Med 24:1878-1892 (2020). PubMed: 31881124
- Jiao J et al. AID assists DNMT1 to attenuate BCL6 expression through DNA methylation in diffuse large B-cell lymphoma cell lines. Neoplasia 22:142-153 (2020). PubMed: 32062068
