Anti-DNAJC16抗体(ab122855)
Key features and details
- Rabbit polyclonal to DNAJC16
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DNAJC16抗体 -
描述
兔多克隆抗体to DNAJC16 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 633-764 of Human DNAJC16 (UniProt Q9Y2G8).
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阳性对照
- Human kidney tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122855于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Contains 1 J domain.
Contains 1 thioredoxin domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23341 Human
- SwissProt: Q9Y2G8 Human
- Unigene: 655410 Human
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别名
- DJC16_HUMAN antibody
- DnaJ homolog subfamily C member 16 antibody
- Dnajc16 antibody
- KIAA0962 antibody
图片
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122855 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. -
ab122855, at a 1/50 dilution, staining DNAJC16 in paraffin embedded Human kidney tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab122855 尚未被引用在任何文献中。