Anti-DNAJB6抗体(ab96539)
Key features and details
- Rabbit polyclonal to DNAJB6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-DNAJB6抗体
参阅全部 DNAJB6 一抗 -
描述
兔多克隆抗体to DNAJB6 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 126 of Human DNAJB6 (NP_490647).
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阳性对照
- HeLaS3 and MOLT4 cell lysates for WB and HeLa cells for IF
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Plays an indispensable role in the organization of KRT8/KRT18 filaments. Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin. Suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Isoform B but not isoform A inhibits huntingtin aggregation. Has a stimulatory effect on the ATPase activity of HSP70 in a dose-dependent and time-dependent manner and hence acts as a co-chaperone of HSP70. Also reduces cellular toxicity and caspase-3 activity. -
组织特异性
Widely expressed. Highest levels in testis and brain, and lower levels in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver and leukocytes. In testis, expressed in germ cells in the earlier stages of differentiation pathway as well as in spermatids. In brain, expressed at a higher level in hippocampus and thalamus and a lower level in amygdala, substantia nigra, corpus callosum and caudate nucleus. -
疾病相关
Limb-girdle muscular dystrophy 1E -
序列相似性
Contains 1 J domain. -
结构域
The antiaggregation activity of isoform B resides in the serine-rich region and the C-terminus. -
细胞定位
Cytoplasm, perinuclear region. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. - Information by UniProt
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数据库链接
- Entrez Gene: 282215 Cow
- Entrez Gene: 10049 Human
- Entrez Gene: 23950 Mouse
- Entrez Gene: 362293 Rat
- Omim: 611332 Human
- SwissProt: Q0III6 Cow
- SwissProt: O75190 Human
- SwissProt: O54946 Mouse
see all -
别名
- DJ4 antibody
- DnaJ antibody
- DnaJ (Hsp40) homolog subfamily B member 6 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab96539 被引用在 3 文献中.
- Qian FY et al. A novel recessive mutation affecting DNAJB6a causes myofibrillar myopathy. Acta Neuropathol Commun 9:23 (2021). PubMed: 33557929
- Palmio J et al. Mutations in the J domain of DNAJB6 cause dominant distal myopathy. Neuromuscul Disord 30:38-46 (2020). PubMed: 31955980
- Ghaoui R et al. Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy. Neurology 86:391-8 (2016). PubMed: 26718575